3,3′,5,5′-Tetramethylbenzidine dihydrochloride dihydrate (CAS 207738-08-7)
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3,3',5,5'-Tetramethylbenzidine dihydrochloride dihydrate (TMB) is an aromatic amine that undergoes oxidation by the higher oxidation states of heme peroxidases (compounds I and II) thereby serving as a reducing co-substrate. One electron oxidation of TMB results in a radical cation that creates a charge-transfer complex with the unoxidized compound. This charge transfer complex absorbs at 652 nm (ε = 39,000). The completely oxidized form (diimine) absorbs at 450 nm (ε = 59,000) and is formed by two sequential one-electron oxidations of TMB. Thus the stoichiometry of oxidation is 0.5 mole charge transfer complex (λmax = 652 nm) or 1 mole of diimine (λmax = 450 nm) formed (or TMB oxidized) per mole of hydroperoxide reduced by the peroxidase.
3,3′,5,5′-Tetramethylbenzidine dihydrochloride dihydrate (CAS 207738-08-7) References
1 Josephy, P.D., Eling, T., Mason, R.P. The horseradish peroxidase-catalyzed oxidation of 3,5,3′,5′-tetramethylbenzidine. Free radical and charge-transfer complex intermediates. J Biol Chem 257 3669-3675 (1982). 2 Marquez, L.A., Dunford, H.B. MechanismOrdering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
3,3′,5,5′-Tetramethylbenzidine dihydrochloride dihydrate, 100 mg | sc-205527 | 100 mg | $41.00 | |||
3,3′,5,5′-Tetramethylbenzidine dihydrochloride dihydrate, 1 g | sc-205527A | 1 g | $88.00 |