Molecular structure of 3,3′,5,5′-Tetramethylbenzidine, CAS Number: 54827-17-7
3,3′,5,5′-Tetramethylbenzidine (CAS 54827-17-7)
See product citations (3)
Alternate Names:
TMB substrate; BM blue; Sure Blue TMB
Application:
3,3′,5,5′-Tetramethylbenzidine is a sensitive reagent for the detection of hemoglobin
CAS Number:
54827-17-7
Molecular Weight:
240.34
Molecular Formula:
C16H20N2
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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SDS & Certificate of Analysis
3,3′,5,5′-Tetramethylbenzidine (TMB) is a sensitive and specific reagent for the detection of blood, assay of hemoglobin, and assay of peroxidases. 3,3′,5,5′-Tetramethylbenzidine is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm.
3,3′,5,5′-Tetramethylbenzidine (CAS 54827-17-7) References
- [Mechanisms of peroxidase oxidation of o-dianisidine, 3,3',5,5'-tetramethylbenzidine, and o-phenylenediamine in the presence of sodium dodecyl sulfate]. | Kireĭko, AV., et al. 2006. Bioorg Khim. 32: 80-6. PMID: 16523724
- Double wavelength measurement of 3,3',5,5'-tetramethylbenzidine (TMB) provides a three-fold enhancement of the ELISA measuring range. | Madersbacher, S. and Berger, P. 1991. J Immunol Methods. 138: 121-4. PMID: 1902244
- Investigation of 3,3',5,5'-tetramethylbenzidine as colorimetric substrate for a peroxidatic DNAzyme. | Li, B., et al. 2009. Anal Chim Acta. 651: 234-40. PMID: 19782817
- Highly sensitive and selective colorimetric detection of glutathione based on Ag [I] ion-3,3',5,5'-tetramethylbenzidine (TMB). | Ni, P., et al. 2015. Biosens Bioelectron. 63: 47-52. PMID: 25058938
- Selective extraction and release using (EDTA-Ni)-layered double hydroxide coupled with catalytic oxidation of 3,3',5,5'-tetramethylbenzidine for sensitive detection of copper ion. | Tang, S., et al. 2015. Anal Chim Acta. 885: 106-13. PMID: 26231895
- Selective sensing of submicromolar iron(III) with 3,3',5,5'-tetramethylbenzidine as a chromogenic probe. | Zhang, L. and Du, J. 2016. Spectrochim Acta A Mol Biomol Spectrosc. 158: 24-8. PMID: 26783724
- A simple and novel colorimetric assay for tyrosinase and inhibitor screening using 3,3',5,5'-tetramethylbenzidine as a chromogenic probe. | Lei, C., et al. 2017. Talanta. 175: 457-462. PMID: 28842016
- Colorimetric Sensor Arrays for Antioxidant Discrimination Based on the Inhibition of the Oxidation Reaction between 3,3',5,5'-Tetramethylbenzidine and Hydrogen Peroxides. | Li, X., et al. 2019. ACS Appl Mater Interfaces. 11: 9504-9509. PMID: 30735025
- A system composed of polyethylenimine-capped upconversion nanoparticles, copper(II), hydrogen peroxide and 3,3',5,5'-tetramethylbenzidine for colorimetric and fluorometric determination of glyphosate. | Liu, Z., et al. 2019. Mikrochim Acta. 186: 835. PMID: 31758331
- Rationale of 3,3',5,5'-Tetramethylbenzidine as the Chromogenic Substrate in Colorimetric Analysis. | Zhang, X., et al. 2020. Anal Chem. 92: 12400-12406. PMID: 32794705
- 3,3',5,5'-tetramethylbenzidine as multi-colorimetric indicator of chlorine in water in line with health guideline values. | Palladino, P., et al. 2020. Anal Bioanal Chem. 412: 7861-7869. PMID: 32870352
- A colorimetric method for screening α-glucosidase inhibitors from flavonoids using 3,3',5,5'-tetramethylbenzidine as a chromogenic probe. | Liu, DM., et al. 2021. Colloids Surf B Biointerfaces. 197: 111400. PMID: 33113490
- Integration and Quantitative Visualization of 3,3',5,5'-Tetramethylbenzidine-Probed Enzyme-Linked Immunosorbent Assay-like Signals in a Photothermal Bar-Chart Microfluidic Chip for Multiplexed Immunosensing. | Fu, G., et al. 2021. Anal Chem. 93: 15105-15114. PMID: 34734693
- The use of 3,3',5,5'-tetramethylbenzidine as a peroxidase substrate in microplate enzyme-linked immunosorbent assay. | Goka, AK. and Farthing, MJ. 1987. J Immunoassay. 8: 29-41. PMID: 3558827
- 3,3',5,5' - Tetramethylbenzidine as an Ames test negative chromogen for horse-radish peroxidase in enzyme-immunoassay. | Bos, ES., et al. 1981. J Immunoassay. 2: 187-204. PMID: 7047570
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
3,3′,5,5′-Tetramethylbenzidine, 500 mg | sc-208442 | 500 mg | $37.00 | |||
| US: (Out of Stock: Availability April 2, 2026) | ||||||
3,3′,5,5′-Tetramethylbenzidine, 1 g | sc-208442A | 1 g | $53.00 | |||
3,3′,5,5′-Tetramethylbenzidine, 5 g | sc-208442B | 5 g | $171.00 | |||
3,3′,5,5′-Tetramethylbenzidine, 25 g | sc-208442C | 25 g | $515.00 | |||
3,3′,5,5′-Tetramethylbenzidine, 100 g | sc-208442D | 100 g | $916.00 | |||
3,3′,5,5′-Tetramethylbenzidine, 250 g | sc-208442E | 250 g | $2271.00 | |||