Date published: 2026-5-6

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2′-O-Methylcytidine (CAS 2140-72-9)

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Application:
2′-O-Methylcytidine is a cytidine analog
CAS Number:
2140-72-9
Purity:
≥98%
Molecular Weight:
257.24
Molecular Formula:
C10H15N3O5
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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2′-O-Methylcytidine (2′-OMe-C) stands as a modified nucleoside that finds extensive utility in diverse scientific research applications. Serving as a modified version of cytidine, a nucleoside inherent in DNA and RNA, 2′-O-Methylcytidine assumes a pivotal role in biochemical, physiological, and genetic investigations. Its application spans studies exploring the impact of modified nucleosides on gene expression and protein-nucleic acid interactions. Within the realm of scientific research, 2′-O-Methylcytidine proves indispensable. It serves as useful for investigating the effects of modified nucleosides on vital processes such as DNA replication, RNA stability, DNA repair, and gene regulation. By employing 2′-O-Methylcytidine, researchers can unravel the intricate connections between modified nucleosides and these fundamental aspects of genetic and molecular biology. Although the precise mechanism of action for 2′-O-Methylcytidine remains not fully elucidated, it is widely postulated that the modified nucleoside selectively binds to specific sites on DNA and RNA. This binding event subsequently triggers structural alterations in the nucleic acid, leading to consequential changes. These changes can manifest as variations in gene expression or alterations in the stability of the nucleic acid itself.


2′-O-Methylcytidine (CAS 2140-72-9) References

  1. Catabolism of the methyl derivatives of adenosine and cytidine in staphylococci.  |  Krasuski, A. and Marlewski, M. 1999. Microbios. 98: 149-57. PMID: 10464951
  2. Identification of a modified nucleoside in Escherichia coli tRNA1Ser as 2'-O-methylcytidine.  |  Yamada, Y. and Ishikura, H. 1975. Biochim Biophys Acta. 402: 285-7. PMID: 1100116
  3. Crystal structure and mutational study of a unique SpoU family archaeal methylase that forms 2'-O-methylcytidine at position 56 of tRNA.  |  Kuratani, M., et al. 2008. J Mol Biol. 375: 1064-75. PMID: 18068186
  4. Osmium Tag for Post-transcriptionally Modified RNA.  |  Debnath, TK. and Okamoto, A. 2018. Chembiochem. 19: 1653-1656. PMID: 29799158
  5. Identification and Quantification of Modified Nucleosides in Saccharomyces cerevisiae mRNAs.  |  Tardu, M., et al. 2019. ACS Chem Biol. 14: 1403-1409. PMID: 31243956
  6. Structural and Energetic Effects of O2'-Ribose Methylation of Protonated Pyrimidine Nucleosides.  |  He, CC., et al. 2019. J Am Soc Mass Spectrom. 30: 2318-2334. PMID: 31435890
  7. Metabolic Drug Response Phenotyping in Colorectal Cancer Organoids by LC-QTOF-MS.  |  Neef, SK., et al. 2020. Metabolites. 10: PMID: 33271860
  8. Development of a highly sensitive method for the quantitative analysis of modified nucleosides using UHPLC-UniSpray-MS/MS.  |  Kogaki, T., et al. 2021. J Pharm Biomed Anal. 197: 113943. PMID: 33601155
  9. Serum metabolomics analysis for the progression of esophageal squamous cell carcinoma.  |  Li, X., et al. 2021. J Cancer. 12: 3190-3197. PMID: 33976728
  10. Mass Spectrometry-Based Targeted Serum Monomethylated Ribonucleosides Profiling for Early Detection of Breast Cancer.  |  Fang, Z., et al. 2021. Front Mol Biosci. 8: 741603. PMID: 34513933
  11. Simultaneous Determination of Methylated Nucleosides by HILIC-MS/MS Revealed Their Alterations in Urine from Breast Cancer Patients.  |  Fang, Z., et al. 2022. Metabolites. 12: PMID: 36295875
  12. 2'-O-methylation of adenosine, guanosine, uridine, and cytidine in RNA of isolated rat liver nuclei.  |  al-Arif, A. and Sporn, MB. 1972. Proc Natl Acad Sci U S A. 69: 1716-9. PMID: 4340155
  13. Synthesis of 2'-O-methyluridine, 2'-O-methylcytidine and their relating compounds.  |  Furukawa, Y., et al. 1965. Chem Pharm Bull (Tokyo). 13: 1273-8. PMID: 5864715

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

2′-O-Methylcytidine, 5 mg

sc-220825
5 mg
$80.00