Molecular structure of 2′,7′-Dichlorodihydrofluorescein Diacetate, CAS Number: 4091-99-0
2′,7′-Dichlorodihydrofluorescein Diacetate (CAS 4091-99-0)
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Alternate Names:
DCFH-DA; DCFH; 2′,7′-Dichlorofluorescin diacetate
Application:
2′,7′-Dichlorodihydrofluorescein Diacetate is a cell-permeable fluorogenic ROS probe
CAS Number:
4091-99-0
Molecular Weight:
487.29
Molecular Formula:
C24H16Cl2O7
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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2′,7′-Dichlorofluorescin Diacetate is a cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) and nitric oxide (•NO) and for the determination of the degree of overall oxidative stress. 2′,7′-Dichlorodihydrofluorescein Diacetate is a soluble white crystalline solid that has found extensive application in scientific research. Its versatility lies in its ability to investigate the impact of diverse drugs on cell cultures in vitro. Moreover, it serves as useful in laboratory experiments, facilitating the study of drug effects on various biological systems such as enzymes, receptors, and proteins. Notably, this compound exhibits inhibitory properties against specific bacteria and demonstrates antioxidant activity. Furthermore, it has demonstrated interactions with certain proteins in the body, potentially influencing their functionality and behavior.
2′,7′-Dichlorodihydrofluorescein Diacetate (CAS 4091-99-0) References
- Evidence for free radical formation during the oxidation of 2'-7'-dichlorofluorescin to the fluorescent dye 2'-7'-dichlorofluorescein by horseradish peroxidase: possible implications for oxidative stress measurements. | Rota, C., et al. 1999. Free Radic Biol Med. 27: 873-81. PMID: 10515592
- Activity of artichoke leaf extract on reactive oxygen species in human leukocytes. | Pérez-García, F., et al. 2000. Free Radic Res. 33: 661-5. PMID: 11200096
- Is the neutrophil reactive oxygen species production measured by luminol and lucigenin chemiluminescence intra or extracellular? Comparison with DCFH-DA flow cytometry and cytochrome c reduction. | Caldefie-Chézet, F., et al. 2002. Clin Chim Acta. 319: 9-17. PMID: 11922918
- Flow cytometric measurement of reactive oxygen species production by normal and thalassaemic red blood cells. | Amer, J., et al. 2003. Eur J Haematol. 70: 84-90. PMID: 12581189
- Evaluation of the probes 2',7'-dichlorofluorescin diacetate, luminol, and lucigenin as indicators of reactive species formation. | Myhre, O., et al. 2003. Biochem Pharmacol. 65: 1575-82. PMID: 12754093
- Curcumin inhibits UV irradiation-induced oxidative stress and apoptotic biochemical changes in human epidermoid carcinoma A431 cells. | Chan, WH., et al. 2003. J Cell Biochem. 90: 327-38. PMID: 14505349
- Active oxygen chemistry within the liposomal bilayer. Part IV: Locating 2',7'-dichlorofluorescein (DCF), 2',7'-dichlorodihydrofluorescein (DCFH) and 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) in the lipid bilayer. | Afri, M., et al. 2004. Chem Phys Lipids. 131: 123-33. PMID: 15210370
- Nox4 is critical for hypoxia-inducible factor 2-alpha transcriptional activity in von Hippel-Lindau-deficient renal cell carcinoma. | Maranchie, JK. and Zhan, Y. 2005. Cancer Res. 65: 9190-3. PMID: 16230378
- The oxidation of 2',7'-dichlorofluorescin to reactive oxygen species: a self-fulfilling prophesy? | Bonini, MG., et al. 2006. Free Radic Biol Med. 40: 968-75. PMID: 16540392
- 2,3,7,8-tetrachlorodibenzo-p-dioxin increases reactive oxygen species production in human endothelial cells via induction of cytochrome P4501A1. | Kopf, PG. and Walker, MK. 2010. Toxicol Appl Pharmacol. 245: 91-9. PMID: 20171976
- Effects of H(2)O(2) exposure on human sperm motility parameters, reactive oxygen species levels and nitric oxide levels. | du Plessis, SS., et al. 2010. Andrologia. 42: 206-10. PMID: 20500750
- A Caco-2 cell-based quantitative antioxidant activity assay for antioxidants. | Wan, H., et al. 2015. Food Chem. 175: 601-8. PMID: 25577125
- Real time monitoring and quantification of reactive oxygen species in breast cancer cell line MCF-7 by 2',7'-dichlorofluorescin diacetate (DCFDA) assay. | Figueroa, D., et al. 2018. J Pharmacol Toxicol Methods. 94: 26-33. PMID: 29630935
- Syzygium cumini extract induced reactive oxygen species-mediated apoptosis in human oral squamous carcinoma cells. | Ezhilarasan, D., et al. 2019. J Oral Pathol Med. 48: 115-121. PMID: 30451321
- Determination of nitric oxide generation in mammalian neurons using dichlorofluorescin diacetate and flow cytometry. | Gabriel, C., et al. 1997. J Pharmacol Toxicol Methods. 38: 93-8. PMID: 9403780
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
2′,7′-Dichlorodihydrofluorescein Diacetate, 100 mg | sc-359840 | 100 mg | $104.00 | |||
2′,7′-Dichlorodihydrofluorescein Diacetate, 250 mg | sc-359840A | 250 mg | $190.00 | |||
2′,7′-Dichlorodihydrofluorescein Diacetate, 500 mg | sc-359840B | 500 mg | $328.00 |