2prime,7prime-bis(2-Carboxyethyl)-5(6)-carboxyfluorescein CAS: 85138-49-4
MF: C27H20O11
MW: 520.44
Fluorescent cytoplasmic pH indicator.

2′,7′-bis(2-Carboxyethyl)-5(6)-carboxyfluorescein (CAS 85138-49-4)

  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 148ms
  • REVIEWS, PRODUCT
  • bvseo-msg: The resource to the URL or file is currently unavailable.;
Alternate Names: BCECF
Application: 2′,7′-bis(2-Carboxyethyl)-5(6)-carboxyfluorescein is Fluorescent cytoplasmic pH indicator
CAS Number: 85138-49-4
Purity: ≥90%
Molecular Weight: 520.44
Molecular Formula: C27H20O11
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
Submit a review for this product and receive 15 CruzCredits

2',7'-bis(2-Carboxyethyl)-5(6)-carboxyfluorescein (BCECF) is a widely used fluorescent indicator for the measurement of cytoplasmic pH in cells. BCECF has been used to: observe regulation of basal acid secretion by glycogen synthase kinase 3, identify enzymatic activity, determine transport activity in human erythrocytes, observe Na+/H+ exchange activity and regulation of gastric acid release.


References

1. Rink, T.J., et al. 1982. J. Cell Biol. 95: 189-196. PMID: 6815204
2. Rotte, A., et al. 2010. Biochem. Biophys. Res. Commun. 398: 677-682. PMID: 20609358
3. Rotte, A., et al. 2010. Cell. Physiol. Biochem. 25: 695-704. PMID: 20511715
4. Wesołowska, O., et al. 2010. Eur. J. Pharmacol. 644: 32-40. PMID: 20633549
5. Rotte, A., et al. 2010. J Gastroenterol. 45: 1022-1032. PMID: 20552232
6. Foladori, P., et al. 2010. Water Res. 44: 3807-3818. PMID: 20537673

Appearance :
Powder
Physical State :
Solid
Solubility :
Soluble in water (slightly), DMSO, DMF, and methanol.
Storage :
Store at -20° C
Boiling Point :
936.23° C at 760 mmHg (Predicted)
Density :
1.68 g/cm3 (Predicted)
Refractive Index :
n20D 1.74 (Predicted)
Fluoresence :
λex 503 ± 3 nm, λem 527 ± 4 nm in Buffer pH 9; λex 482 nm, λem 520 nm in 0.1 M Tris pH 7.0
pK Values :
pKa: 6.97
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
PubChem CID :
4241719
MDL Number :
MFCD00036970
Beilstein Registry :
8375770
SMILES :
C1=CC(=C(C=C1C(=O)O)C2=C3C=C(C(=O)C=C3OC4=CC(=C(C=C42)CCC(=O)O)O)CCC(=O)O)C(=O)O

Download SDS (MSDS)

Certificate of Analysis

Adobe Acrobat Reader is required to reliably view,
print and comment on PDF documents

2prime,7prime-bis(2-Carboxyethyl)-5(6)-carboxyfluorescein (CAS 85138-49-4) Product Citations

See how others have used 2prime,7prime-bis(2-Carboxyethyl)-5(6)-carboxyfluorescein (CAS 85138-49-4). Click on the entry to view the PubMed entry .

Citations 1 to 3 of 3 total

PMID: # 21545783  Di Bernardo, J. et al. 2011. Anal. Biochem. 415: 32-38.

PMID: # 11749926  Effros, RM. et al. 2001. Am. J. Med. 111 Suppl 8A: 56S-59S.

PMID: # 11600438  Galietta, LV. et al. 2001. Am. J. Physiol., Cell Physiol. 281: C1734-C1742.

Citations 1 to 3 of 3 total
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 138ms
  • QUESTIONS, PRODUCT
  • bvseo-msg: The resource to the URL or file is currently unavailable.;
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 46ms
  • REVIEWS, PRODUCT
  • bvseo-msg: The resource to the URL or file is currently unavailable.;

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2020 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.