26S Proteasome p48A Antibody (1B8): sc-65749
- 26S Proteasome p48A Antibody (1B8) is a mouse monoclonal IgG1 κ 26S Proteasome p48A antibody provided at 200 µg/ml
- raised against 26S Proteasome purified from embryos of Drosophila melanogaster origin
- 26S Proteasome p48A Antibody (1B8) is recommended for detection of p48A subunit of the 19S regulatory base complex of the 26S Proteasome of d origin by WB and IP
- Anti-26S Proteasome p48A Antibody (1B8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP is the preferred secondary detection reagent for 26S Proteasome p48A Antibody (1B8) for WB applications. This reagent is now offered in a bundle with 26S Proteasome p48A Antibody (1B8) (see ordering information below).
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26S Proteasome p48A Antibody (1B8) is a mouse monoclonal IgG1 kappa light chain antibody that detects 26S Proteasome p48A protein of Drosophila melanogaster origin by western blotting (WB) and immunoprecipitation (IP). Anti-26S Proteasome p48A antibody (1B8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. 26S proteasome is a crucial multi-subunit complex responsible for degrading ubiquitinated proteins, a process vital for maintaining cellular homeostasis and regulating numerous biological pathways, including cell cycle progression and stress responses. This complex comprises over 30 distinct subunits, with 26S proteasome p48A, also known as Rpt3 in yeast and S6 in humans, serving as one of six ATPase base subunits within the 19S regulatory complex. 19S regulatory complex plays a key role in recognizing ubiquitinated substrates, removing ubiquitin tags, and facilitating translocation into the 20S core for degradation. Assembly of 26S proteasome is ATP-dependent, and due to large size, inherently unstable, often dissociating into subcomplexes, which include a 20S core and two 19S regulatory complexes. 26S proteasome p48A is essential for recruiting p30 (Rpn12/S14) subunit of the 19S lid, highlighting importance in overall function and stability of proteasome complex.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
26S Proteasome p48A Antibody (1B8) References:
- Assembly of the Drosophila 26 S proteasome is accompanied by extensive subunit rearrangements. | Kurucz, E., et al. 2002. Biochem J. 365: 527-36. PMID: 11945175
- Deletion of proteasomal subunit S5a/Rpn10/p54 causes lethality, multiple mitotic defects and overexpression of proteasomal genes in Drosophila melanogaster. | Szlanka, T., et al. 2003. J Cell Sci. 116: 1023-33. PMID: 12584246
- The HALTED ROOT gene encoding the 26S proteasome subunit RPT2a is essential for the maintenance of Arabidopsis meristems. | Ueda, M., et al. 2004. Development. 131: 2101-11. PMID: 15073153
- ATP hydrolysis-dependent disassembly of the 26S proteasome is part of the catalytic cycle. | Babbitt, SE., et al. 2005. Cell. 121: 553-565. PMID: 15907469
- The proteasome regulatory particle alters the SAGA coactivator to enhance its interactions with transcriptional activators. | Lee, D., et al. 2005. Cell. 123: 423-36. PMID: 16269334
- The proteasome restricts permissive transcription at tissue-specific gene loci in embryonic stem cells. | Szutorisz, H., et al. 2006. Cell. 127: 1375-88. PMID: 17190601
- Mass spectrometric characterization of the affinity-purified human 26S proteasome complex. | Wang, X., et al. 2007. Biochemistry. 46: 3553-65. PMID: 17323924
- Aging perturbs 26S proteasome assembly in Drosophila melanogaster. | Vernace, VA., et al. 2007. FASEB J. 21: 2672-82. PMID: 17413001
- Rpn13p and Rpn14p are involved in the recognition of ubiquitinated Gcn4p by the 26S proteasome. | Seong, KM., et al. 2007. FEBS Lett. 581: 2567-73. PMID: 17499717
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
26S Proteasome p48A Antibody (1B8) | sc-65749 | 200 µg/ml | $322.00 | |||
26S Proteasome p48A Antibody (1B8): m-IgG1 BP-HRP Bundle | sc-531648 | 200 µg Ab; 20 µg BP | $361.00 | |||
26S Proteasome p48A Antibody (1B8) AC | sc-65749 AC | 500 µg/ml, 25% agarose | $424.00 | |||
26S Proteasome p48A Antibody (1B8) HRP | sc-65749 HRP | 200 µg/ml | $322.00 | |||
26S Proteasome p48A Antibody (1B8) FITC | sc-65749 FITC | 200 µg/ml | $336.00 | |||
26S Proteasome p48A Antibody (1B8) PE | sc-65749 PE | 200 µg/ml | $349.00 | |||
26S Proteasome p48A Antibody (1B8) Alexa Fluor® 488 | sc-65749 AF488 | 200 µg/ml | $364.00 | |||
26S Proteasome p48A Antibody (1B8) Alexa Fluor® 546 | sc-65749 AF546 | 200 µg/ml | $364.00 | |||
26S Proteasome p48A Antibody (1B8) Alexa Fluor® 594 | sc-65749 AF594 | 200 µg/ml | $364.00 | |||
26S Proteasome p48A Antibody (1B8) Alexa Fluor® 647 | sc-65749 AF647 | 200 µg/ml | $364.00 | |||
26S Proteasome p48A Antibody (1B8) Alexa Fluor® 680 | sc-65749 AF680 | 200 µg/ml | $364.00 | |||
26S Proteasome p48A Antibody (1B8) Alexa Fluor® 790 | sc-65749 AF790 | 200 µg/ml | $364.00 |