26S Proteasome p42C Antibody (9E3): sc-65752
- 26S Proteasome p42C Antibody (9E3) is a mouse monoclonal IgG1 κ 26S Proteasome p42C antibody provided at 200 µg/ml
- raised against 26S Proteasome purified from embryos of Drosophila melanogaster origin
- 26S Proteasome p42C Antibody (9E3) is recommended for detection of p42C subunit of the 19S regulatory base complex of the 26S Proteasome of d origin by WB and IP
- Anti-26S Proteasome p42C Antibody (9E3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for 26S Proteasome p42C Antibody (9E3). This work is in progress.
QUICK LINKS
SEE ALSO...
26S Proteasome p42C Antibody (9E3) is a mouse monoclonal IgG1 kappa light chain antibody that detects 26S Proteasome p42C protein of Drosophila melanogaster origin by western blotting (WB) and immunoprecipitation (IP). Anti-26S Proteasome p42C antibody (9E3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. 26S proteasome is a crucial multi-subunit complex responsible for degrading ubiquitinated proteins, a process vital for maintaining cellular homeostasis and regulating numerous biological pathways, including cell cycle progression, apoptosis, and responses to oxidative stress. 26S proteasome p42C, also known as Rpt6 in yeast and S8 in humans, is one of six ATPase base subunits of 19S regulatory complex, which plays a key role in recognizing ubiquitinated substrates, removing ubiquitin chains, and facilitating protein translocation into 20S core for degradation. Assembly of 26S proteasome is ATP-dependent, and due to large size, prone to disassociation into subcomplexes, which can impact functionality. Understanding dynamics and interactions of 26S proteasome p42C is essential for elucidating roles in cellular regulation and potential implications in various diseases, including cancer and neurodegenerative disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
26S Proteasome p42C Antibody (9E3) References:
- Assembly of the Drosophila 26 S proteasome is accompanied by extensive subunit rearrangements. | Kurucz, E., et al. 2002. Biochem J. 365: 527-36. PMID: 11945175
- Deletion of proteasomal subunit S5a/Rpn10/p54 causes lethality, multiple mitotic defects and overexpression of proteasomal genes in Drosophila melanogaster. | Szlanka, T., et al. 2003. J Cell Sci. 116: 1023-33. PMID: 12584246
- The HALTED ROOT gene encoding the 26S proteasome subunit RPT2a is essential for the maintenance of Arabidopsis meristems. | Ueda, M., et al. 2004. Development. 131: 2101-11. PMID: 15073153
- ATP hydrolysis-dependent disassembly of the 26S proteasome is part of the catalytic cycle. | Babbitt, SE., et al. 2005. Cell. 121: 553-565. PMID: 15907469
- Mass spectrometric characterization of the affinity-purified human 26S proteasome complex. | Wang, X., et al. 2007. Biochemistry. 46: 3553-65. PMID: 17323924
- Aging perturbs 26S proteasome assembly in Drosophila melanogaster. | Vernace, VA., et al. 2007. FASEB J. 21: 2672-82. PMID: 17413001
- Rpn13p and Rpn14p are involved in the recognition of ubiquitinated Gcn4p by the 26S proteasome. | Seong, KM., et al. 2007. FEBS Lett. 581: 2567-73. PMID: 17499717
- Automated cryoelectron microscopy of 'single particles' applied to the 26S proteasome. | Nickell, S., et al. 2007. FEBS Lett. 581: 2751-6. PMID: 17531228
- Cloning and characterization of Pros45, the Drosophila SUG1 proteasome subunit homolog. | Cheng, L., et al. 1998. Mol Gen Genet. 259: 13-20. PMID: 9738875
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
26S Proteasome p42C Antibody (9E3) | sc-65752 | 200 µg/ml | $322.00 | |||
26S Proteasome p42C Antibody (9E3) AC | sc-65752 AC | 500 µg/ml, 25% agarose | $424.00 | |||
26S Proteasome p42C Antibody (9E3) HRP | sc-65752 HRP | 200 µg/ml | $322.00 | |||
26S Proteasome p42C Antibody (9E3) FITC | sc-65752 FITC | 200 µg/ml | $336.00 | |||
26S Proteasome p42C Antibody (9E3) PE | sc-65752 PE | 200 µg/ml | $349.00 | |||
26S Proteasome p42C Antibody (9E3) Alexa Fluor® 488 | sc-65752 AF488 | 200 µg/ml | $364.00 | |||
26S Proteasome p42C Antibody (9E3) Alexa Fluor® 546 | sc-65752 AF546 | 200 µg/ml | $364.00 | |||
26S Proteasome p42C Antibody (9E3) Alexa Fluor® 594 | sc-65752 AF594 | 200 µg/ml | $364.00 | |||
26S Proteasome p42C Antibody (9E3) Alexa Fluor® 647 | sc-65752 AF647 | 200 µg/ml | $364.00 | |||
26S Proteasome p42C Antibody (9E3) Alexa Fluor® 680 | sc-65752 AF680 | 200 µg/ml | $364.00 | |||
26S Proteasome p42C Antibody (9E3) Alexa Fluor® 790 | sc-65752 AF790 | 200 µg/ml | $364.00 |