Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
20S Proteasome β5 CRISPR/Cas9 KO Plasmid (h): sc-402449
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- 20S Proteasome β5 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the 20S Proteasome β5 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: 20S Proteasome β5 Antibody (A-10): sc-393931
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
20S Proteasome β5 CRISPR/Cas9 KO Plasmid (h) | sc-402449 | 20 µg | $397.00 |
Overview
PSMB5 encodes the β5 catalytic subunit of the 20S core proteasome, which provides the chymotrypsin-like activity essential for ATP-dependent protein degradation by the ubiquitin–proteasome system. This proteolytic pathway regulates protein quality control, antigen processing for MHC class I presentation, and turnover of key cell-cycle and stress-response regulators. Altered proteasome function has been linked to dysregulated proteostasis, oxidative and ER stress signaling, and changes in NF-κB and apoptotic pathways. PSMB5 is also studied in contexts such as proliferative signaling and responses to proteasome inhibition, where β5 activity can influence cellular sensitivity and resistance phenotypes.
20S Proteasome β5 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PSMB5 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PSMB5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PSMB5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish 20S Proteasome β5 protein expression.
This CRISPR knockout system enables efficient generation of PSMB5-deficient cell models for investigation of 20S Proteasome β5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PSMB5 exon(s) critical for 20S Proteasome β5 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PSMB5 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by 20S Proteasome β5 CRISPR/Cas9 KO Plasmid (h) and 20S Proteasome β5 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PSMB5 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by 20S Proteasome β5 HDR Plasmid (h) and 20S Proteasome β5 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PSMB5 homology arms to support homology-directed repair at defined PSMB5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.