20S Proteasome α3 Antibody (D-6): sc-166206
- 20S Proteasome α3 Antibody (D-6) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against amino acids 131-255 mapping at the C-terminus of 20S Proteasome α3 of human origin
- recommended for detection of 20S Proteasome α3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including bovine
- m-IgG1 BP-HRP is the preferred secondary detection reagent for 20S Proteasome α3 Antibody (D-6) for WB and IHC(P) applications. This reagent is now offered in a bundle with 20S Proteasome α3 Antibody (D-6) (see ordering information below).
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20S Proteasome α3 Antibody (D-6) is a mouse monoclonal IgG1 antibody that detects PSMA3 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). PSMA3 is a crucial component of the 26S proteasome, which plays a vital role in maintaining cellular homeostasis by degrading unnecessary or damaged proteins through proteolysis. This process is essential for regulating various cellular functions, including cell cycle, signal transduction, and response to oxidative stress. The 20S core particle, where PSMA3 resides, forms a hollow cylinder that allows selective entry of substrates, ensuring only targeted proteins are processed. PSMA3 and other α subunits serve as structural components that provide stability to the proteasome and facilitate docking of regulatory particles, which are critical for recognition and unfolding of substrates prior to degradation. Anti-PSMA3 antibody (D-6) enables researchers to study protein degradation dynamics and implications in various diseases, including cancer and neurodegenerative disorders, where proteasome function is often compromised.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
20S Proteasome α3 Antibody (D-6) References:
- A multimeric assembly factor controls the formation of alternative 20S proteasomes. | Kusmierczyk, AR., et al. 2008. Nat Struct Mol Biol. 15: 237-44. PMID: 18278055
- Dopaminergic therapies modulate the T-CELL proteome of patients with Parkinson's disease. | Alberio, T., et al. 2012. IUBMB Life. 64: 846-52. PMID: 22815142
- Reconfiguration of the proteasome during chaperone-mediated assembly. | Park, S., et al. 2013. Nature. 497: 512-6. PMID: 23644457
- Assembly of an Evolutionarily Conserved Alternative Proteasome Isoform in Human Cells. | Padmanabhan, A., et al. 2016. Cell Rep. 14: 2962-74. PMID: 26997268
- The insulin-degrading enzyme is an allosteric modulator of the 20S proteasome and a potential competitor of the 19S. | Sbardella, D., et al. 2018. Cell Mol Life Sci. 75: 3441-3456. PMID: 29594388
- Cationic porphyrins are tunable gatekeepers of the 20S proteasome. | Santoro, AM., et al. 2016. Chem Sci. 7: 1286-1297. PMID: 29910886
- Reduced chronic restraint stress in mice overexpressing hyperactive proteasomes in the forebrain. | Kim, JH., et al. 2020. Mol Brain. 13: 4. PMID: 31931843
- Post-Translational Modifications of Extracellular Proteasome. | Tsimokha, AS., et al. 2020. Molecules. 25: PMID: 32752045
- Hsp70 and Hsp110 Chaperones Promote Early Steps of Proteasome Assembly. | Matias, AC., et al. 2022. Biomolecules. 13: PMID: 36671396
- Normal Proteasome Function Is Needed to Prevent Kidney Graft Injury during Cold Storage Followed by Transplantation. | Bhattarai, D., et al. 2024. Int J Mol Sci. 25: PMID: 38396827
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
20S Proteasome α3 Antibody (D-6) | sc-166206 | 200 µg/ml | $316.00 | |||
20S Proteasome α3 Antibody (D-6): m-IgG1 BP-HRP Bundle | sc-542658 | 200 µg Ab; 20 µg BP | $354.00 |