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2-Nitrophenylhydrazine hydrochloride (CAS 6293-87-4)

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Alternate Names:
(2-nitrophenyl)hydrazine hydrochloride; o-Nitrophenylhydrazine hydrochloride; 2-Nitrophenyl Hydrazine Hydrochloride
CAS Number:
6293-87-4
Purity:
≥97%
Molecular Weight:
189.6
Molecular Formula:
C6H7N3O2•HCl
Supplemental Information:
This is classified as a Dangerous Good for transport and may be subject to additional shipping charges.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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2-Nitrophenylhydrazine hydrochloride is a versatile organic compound in multiple scientific and industrial applications. Its applications span from detecting glucose and assessing enzyme activity to synthesizing aldehydes and ketones. It serves as a reagent for ascertaining the presence of amines and amino acids, and it plays a role as a catalyst in the synthesis of diverse organic compounds. Notably, 2-Nitrophenylhydrazine hydrochloride functions as a reducing agent, operating by reducing nitro groups to amines.


2-Nitrophenylhydrazine hydrochloride (CAS 6293-87-4) References

  1. Measurement of 3-hydroxyisovaleric acid in urine of biotin-deficient infants and mice by HPLC.  |  Watanabe, T., et al. 2005. J Nutr. 135: 615-8. PMID: 15735103
  2. Characteristic long-chain fatty acid of Pleurocybella porrigens.  |  Amakura, Y., et al. 2006. Shokuhin Eiseigaku Zasshi. 47: 178-81. PMID: 16984039
  3. Determination of biotin following derivatization with 2-nitrophenylhydrazine by high-performance liquid chromatography with on-line UV detection and electrospray-ionization mass spectrometry.  |  Yomota, C. and Ohnishi, Y. 2007. J Chromatogr A. 1142: 231-5. PMID: 17234197
  4. Colorimetric determination of some penicillins and cephalosporins with 2-nitrophenylhydrazine hydrochloride.  |  Korany, MA., et al. 1989. Talanta. 36: 1253-7. PMID: 18964900
  5. Determination of small halogenated carboxylic acid residues in drug substances by high performance liquid chromatography-diode array detection following derivatization with nitro-substituted phenylhydrazines.  |  Hou, D., et al. 2016. J Chromatogr A. 1438: 46-56. PMID: 26893023
  6. Validated Spectrophotometric and RP-HPLC-DAD Methods for the Determination of Ursodeoxycholic Acid Based on Derivatization with 2-Nitrophenylhydrazine.  |  El-Kafrawy, DS., et al. 2017. J AOAC Int. 100: 677-685. PMID: 28118581
  7. A colorimetric assay method for measuring d-glutamate cyclase activity.  |  Katane, M., et al. 2020. Anal Biochem. 605: 113838. PMID: 32702438
  8. Reversed-phase ion-pair chromatography of straight- and branched-chain dicarboxylic acids in urine as their 2-nitrophenylhydrazides.  |  Miwa, H. and Yamamoto, M. 1988. Anal Biochem. 170: 301-7. PMID: 3394930
  9. High-performance liquid chromatographic analysis of serum short-chain fatty acids by direct derivatization.  |  Miwa, H. and Yamamoto, M. 1987. J Chromatogr. 421: 33-41. PMID: 3429573
  10. High-performance liquid chromatographic analysis of serum long-chain fatty acids by direct derivatization method.  |  Miwa, H., et al. 1987. J Chromatogr. 416: 237-45. PMID: 3611256
  11. Improved method of determination of biologically important C10:0-C22:6 fatty acids as their 2-nitrophenylhydrazides by reversed-phase high-performance liquid chromatography.  |  Miwa, H. and Yamamoto, M. 1986. J Chromatogr. 351: 275-82. PMID: 3958081
  12. Rapid liquid chromatographic determination of fatty acids as 2-nitrophenylhydrazine derivatives.  |  Miwa, H. and Yamamoto, M. 1996. J AOAC Int. 79: 493-7. PMID: 8920138

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

2-Nitrophenylhydrazine hydrochloride, 5 g

sc-356514
5 g
$62.00

2-Nitrophenylhydrazine hydrochloride, 25 g

sc-356514A
25 g
$247.00