2-Nitrophenyl isocyanate (CAS 3320-86-3)

2-Nitrophenyl isocyanate (2-NPIC) serves as a vital organic compound with diverse applications in both scientific and industrial domains. Derived from phenyl isocyanate, it incorporates a nitro group. Researchers and industries widely employ 2-Nitrophenyl isocyanate in multiple fields of study, including organic synthesis and biochemistry. In the realm of organic synthesis, 2-Nitrophenyl isocyanate takes on the role of a valuable reagent, facilitating the preparation of an array of compounds, such as amines, esters, and amides. Meanwhile, in the realm of biochemistry, it functions as a key reagent for detecting proteins and other biomolecules. The mechanism of action of 2-Nitrophenyl isocyanate involves its participation as a nucleophilic reagent in organic synthesis. Through this process, it reacts with electrophiles, like alkenes and alkynes, giving rise to a novel carbon-nitrogen bond. This specific reaction is referred to as an isocyanate reaction. In the domain of biochemistry, 2-Nitrophenyl isocyanate takes on the role of a hapten, actively binding with proteins and other biomolecules, ultimately leading to the formation of stable complexes. These complexes prove for detection purposes and can be identified using various techniques, such as ELISA and Western blotting.