Molecular structure of 2-Nitrophenyl b-D-galactopyranoside, CAS Number: 369-07-3
2-Nitrophenyl b-D-galactopyranoside (CAS 369-07-3)
Application:
2-Nitrophenyl b-D-galactopyranoside is a β-galactosidase substrate for colorimetric and EIA applications
CAS Number:
369-07-3
Purity:
≥97%
Molecular Weight:
301.20
Molecular Formula:
C12H15NO8
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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SDS & Certificate of Analysis
2-Nitrophenyl-beta-D-galactopyranoside (2-NPG) is an artificially created carbohydrate derivative that originates from lactose, a disaccharide. Its structure consists of glucose and galactose, linked together by a glycosidic bond. 2-Nitrophenyl-beta-D-galactopyranoside serves as a substrate for enzymes involved in carbohydrate metabolism. Once 2-Nitrophenyl-beta-D-galactopyranoside binds to a carbohydrate-binding protein, a glycosidase enzyme can cleave it, resulting in the release of glucose and galactose. Subsequently, these monosaccharides can undergo further metabolic processes facilitated by other enzymes.
2-Nitrophenyl b-D-galactopyranoside (CAS 369-07-3) References
- An enzyme flow immunoassay that uses beta-galactosidase as the label and a cellobiose dehydrogenase biosensor as the label detector. | Burestedt, E., et al. 2000. Anal Chem. 72: 4171-7. PMID: 10994980
- Rapid colorimetric assay of beta-galactosidase and N-acetyl-beta-glucosaminidase in human urine. | Maruhn, D. 1976. Clin Chim Acta. 73: 453-61. PMID: 11913
- Characterization of new β-galactosidase from acidophilic fungus, Teratosphaeria acidotherma AIU BGA-1. | Isobe, K., et al. 2013. J Biosci Bioeng. 116: 293-7. PMID: 23623895
- Novel acidophilic β-galactosidase with high activity at extremely acidic pH region from Teratosphaeria acidotherma AIU BGA-1. | Chiba, S., et al. 2015. J Biosci Bioeng. 120: 263-7. PMID: 25797715
- New alkalophilic β-galactosidase with high activity in alkaline pH region from Teratosphaeria acidotherma AIU BGA-1. | Yamada, M., et al. 2017. J Biosci Bioeng. 123: 15-19. PMID: 27514907
- Mg2+-induced stabilization of β-galactosidase from Bacillus megaterium and its application in the galactosylation of natural products. | Zhou, Y., et al. 2017. Biotechnol Lett. 39: 1175-1181. PMID: 28470628
- Intestinal enzyme delivery: Chitosan/tripolyphosphate nanoparticles providing a targeted release behind the mucus gel barrier. | Leichner, C., et al. 2019. Eur J Pharm Biopharm. 144: 125-131. PMID: 31526841
- Transglycosylating β-d-galactosidase and α-l-fucosidase from Paenibacillus sp. 3179 from a hot spring in East Greenland. | Thøgersen, MS., et al. 2020. Microbiologyopen. 9: e980. PMID: 31868312
- A novel cold-adapted phospho-beta-galactosidase from Bacillus velezensis and its potential application for lactose hydrolysis in milk. | Liu, Y., et al. 2021. Int J Biol Macromol. 166: 760-770. PMID: 33144261
- Overexpression and characterization of a novel GH4 galactosidase with β-galactosidase activity from Bacillus velezensis SW5. | Li, N., et al. 2021. J Dairy Sci. 104: 9465-9477. PMID: 34127264
- A dual-signal fluorometric-colorimetric sensing platform and visual detection with a smartphone for the determination of β-galactosidase activity based on fluorescence silicon nanoparticles. | Liu, J., et al. 2022. Talanta. 240: 123165. PMID: 34953382
- A GH42 β-galactosidase from Bifidobacterium thermophilum: Biochemical characterization and synthesis of galactosyl glycerol by reverse hydrolysis. | Duan, J., et al. 2022. Biotechnol Appl Biochem.. PMID: 36181448
Substrate of:
beta-Galactosidase, Enzyme, and HRP and AP.Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
2-Nitrophenyl b-D-galactopyranoside, 10 g | sc-283249 | 10 g | $220.00 | |||
2-Nitrophenyl b-D-galactopyranoside, 25 g | sc-283249A | 25 g | $414.00 | |||
2-Nitrophenyl b-D-galactopyranoside, 100 g | sc-283249B | 100 g | $618.00 |