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2-Nitro-5-thiocyanatobenzoic acid (CAS 30211-77-9)

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Alternate Names:
NTCB
Application:
2-Nitro-5-thiocyanatobenzoic acid is a hydratase inhibitor
CAS Number:
30211-77-9
Purity:
≥98%
Molecular Weight:
224.19
Molecular Formula:
C8H4N2O4S
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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2-Nitro-5-thiocyanatobenzoic acid is used as a reagent in chemical research, where its reactivity is harnessed in the synthesis of complex organic molecules. It often serves as a precursor in the preparation of dyes and pigments due to its ability to undergo various chemical transformations. Additionally, its properties make it suitable for use in the development of sensors and assays, where it can function as an analytical tool for the detection of specific substances. In the study of protein labeling, 2-Nitro-5-thiocyanatobenzoic acid is utilized for the conjugation of labels to proteins, facilitating the tracking and quantification of these biomolecules in different assays. Researchers also explore its applications in materials science for the creation of functional surfaces that can interact with biological molecules.


2-Nitro-5-thiocyanatobenzoic acid (CAS 30211-77-9) References

  1. Identification of alternative products and optimization of 2-nitro-5-thiocyanatobenzoic acid cyanylation and cleavage at cysteine residues.  |  Tang, HY. and Speicher, DW. 2004. Anal Biochem. 334: 48-61. PMID: 15464952
  2. Localization of a substrate binding domain of the human reduced folate carrier to transmembrane domain 11 by radioaffinity labeling and cysteine-substituted accessibility methods.  |  Hou, Z., et al. 2005. J Biol Chem. 280: 36206-13. PMID: 16115875
  3. Scrapie prion protein structural constraints obtained by limited proteolysis and mass spectrometry.  |  Sajnani, G., et al. 2008. J Mol Biol. 382: 88-98. PMID: 18621059
  4. Site-Specific Conversion of Cysteine in a Protein to Dehydroalanine Using 2-Nitro-5-thiocyanatobenzoic Acid.  |  Qiao, Y., et al. 2021. Molecules. 26: PMID: 33947165
  5. A novel procedure for the preparation of biologically active recombinant peptides using a cyanylation reaction.  |  Koyama, N., et al. 1994. J Biotechnol. 32: 273-81. PMID: 7764719
  6. Identification and localization of caldesmon in cardiac muscle.  |  Scott-Woo, GC., et al. 1998. Biochem J. 334 (Pt 1): 161-70. PMID: 9693116
  7. The catalytic subunit of the cAMP-dependent protein kinase of ovine sperm flagella has a unique amino-terminal sequence.  |  San Agustin, JT., et al. 1998. J Biol Chem. 273: 24874-83. PMID: 9733793
  8. Alternative products in the reaction of 2-nitro-5-thiocyanatobenzoic acid with thiol groups.  |  Price, NC. 1976. Biochem J. 159: 177-80. PMID: 999637

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

2-Nitro-5-thiocyanatobenzoic acid, 250 mg

sc-230585
250 mg
$108.00

2-Nitro-5-thiocyanatobenzoic acid, 5 g

sc-230585A
5 g
$1457.00

2-Nitro-5-thiocyanatobenzoic acid, 25 g

sc-230585B
25 g
$7283.00

2-Nitro-5-thiocyanatobenzoic acid, 50 g

sc-230585C
50 g
$14045.00

Can I make stock solution in DMSO or ethanol, then add it to protein lysates for protein fragmentation?

Asked by: szhao55
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Answered by: Technical Support
Date published: 2021-07-22
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Rated 5 out of 5 by from ScottScott-Woo, GC. et al. (PubMed 9693116) used 2-Nitro-5-thiocyanatobenzoic acid and chymotryptic fragments to map the epitopes for the individual monoclonal antibodies to the caldesmon primary structure. -SCBT Publication Review
Date published: 2015-04-13
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2-Nitro-5-thiocyanatobenzoic acid is rated 5.0 out of 5 by 1.
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