2-Isobutyramido Guanosine 2′,3′,5′-Tris(isobutanoate) (CAS 70337-80-3)

2-Isobutyramido Guanosine 2′,3′,5′-Tris(isobutanoate) is a nucleoside derivative designed for molecular and biochemical research focused on understanding nucleic acid synthesis and modification. With isobutyryl groups attached at the 2′, 3′, and 5′ hydroxyl positions and an isobutyramido group at the 2-position of the guanine base, this compound offers distinct structural features that influence its interaction with polymerases and other nucleic acid-binding proteins. The modification enhances its lipophilicity, making it a suitable substrate for studying the intracellular transport of modified nucleosides and their incorporation into nucleic acids. Researchers use this compound to investigate the specificity of nucleoside transporters, gaining insights into how cells handle modified nucleosides compared to their unmodified counterparts. Its incorporation into oligonucleotides can also provide data on how polymerases interact with modified bases during replication and transcription, revealing mechanisms of fidelity and error correction. This tris(isobutanoate) derivative helps researchers explore how base modifications impact the structural stability of nucleic acids, as well as the potential for such modifications to alter the recruitment of repair enzymes. Overall, 2-Isobutyramido Guanosine 2′,3′,5′-Tris(isobutanoate) serves as a tool to deepen the understanding of nucleic acid chemistry and cellular responses to modified nucleosides.