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2-Hydroxy-5-nitrobenzoic acid (CAS 96-97-9)

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Alternate Names:
5-Nitrosalicylic Acid
CAS Number:
96-97-9
Molecular Weight:
183.12
Molecular Formula:
C7H5NO5
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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2-Hydroxy-5-nitrobenzoic acid is a pale yellow crystalline solid soluble in water, ethanol, and multiple polar solvents. It holds various applications in scientific studies and can serve as an HPLC analysis standard, a pH indicator, and a detecting agent for heavy metals. Additionally, 5-NSA can be used to study the oxidation of phenolic compounds, as well as the reaction kinetics of nitrites and nitric oxide. It is an ethylene diamine derivative used in organic synthesis and can engage with nitrogen atoms to create stable complexes. When 2-Hydroxy-5-nitrobenzoic acid reacts with hydrogen, it forms a protonated acid complex, exhibiting higher solubility in water compared to the original molecule. Synchronous fluorescence spectroscopy has shown that 5-nitrosalicylic acid reacts with glycan by hydrogen bonding interactions and stabilizing nitro groups.


2-Hydroxy-5-nitrobenzoic acid (CAS 96-97-9) References

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  2. The decomposition of 2,5-dinitrobenzoic acid by alkali.  |  LANGLEY, WD. 1948. J Am Chem Soc. 70: 1633. PMID: 18915783
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  4. Application of effect-directed analysis to identify mutagenic nitrogenous disinfection by-products of advanced oxidation drinking water treatment.  |  Vughs, D., et al. 2018. Environ Sci Pollut Res Int. 25: 3951-3964. PMID: 27447472
  5. Computational and synthetic approaches for developing Lavendustin B derivatives as allosteric inhibitors of HIV-1 integrase.  |  Agharbaoui, FE., et al. 2016. Eur J Med Chem. 123: 673-683. PMID: 27517812
  6. 3-Hydroxy-2-Nitrobenzoic Acid as a MALDI Matrix for In-Source Decay and Evaluation of the Isomers.  |  Fukuyama, Y., et al. 2018. J Am Soc Mass Spectrom. 29: 2227-2236. PMID: 30062476
  7. Formation of Nitrophenolic Byproducts during Heat-Activated Peroxydisulfate Oxidation in the Presence of Natural Organic Matter and Nitrite.  |  Yang, P., et al. 2019. Environ Sci Technol. 53: 4255-4264. PMID: 30912931
  8. Atmospheric pressure chemical ionization mass spectrometry at low flow rates: Importance of ion source housing.  |  Strmeň, T., et al. 2020. Rapid Commun Mass Spectrom. 34: e8722. PMID: 31912928
  9. In search of therapeutic candidates for HIV/AIDS: rational approaches, design strategies, structure-activity relationship and mechanistic insights.  |  Kumar, D., et al. 2021. RSC Adv. 11: 17936-17964. PMID: 35480193
  10. Stabilization of the DMSO Solvate of 2-Chloro-5-nitrobenzoic acid (Mesalazine Impurity M) by Bifurcated Hydrogen Bonds: Crystallographic, Spectroscopic, Thermal and Computational Studies  |  , et al. (2022). Journal of Chemical Crystallography. volume 52,: pages 276–286.
  11. Permanganate-Induced Nitration of o- and P- Nitrophenol  |  Hassan M. Gomaa, et al. 1971 -. Environmental Letters. Volume 2, Issue 1: Pages 47-49.
  12. Synthesis of Tripodal Phenol Porphyrins: A Modular Approach to Mimic ­Enzymes with a Cross-Linked Tyrosine  |  David Gueyrard, Amandine Didier, Christian Ruzié, Arnaud Bondon, Bernard Boitrel*. 2004(. Synlett. 7):: 1158-1162.
  13. Tetraaqua-bis(3-hydroxy-4-nitrobenzoato) Co(II) and Ni(II) complexes and diaqua-bis(2-hydroxy-4-methoxybenzoato) Zn(II) complex: crystal structure and thorough metal-coordination investigation  |  Jean D'Angelo, et al. 2011. Journal of Coordination Chemistry. Volume 64,- Issue 17: Pages 3110-3122.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

2-Hydroxy-5-nitrobenzoic acid, 25 g

sc-238076
25 g
$56.00