2-Deoxy-D-glucose-tetraacetate (CAS 69515-91-9)

2-Deoxy-D-glucose-tetraacetate is a derivative of 2-deoxy-D-glucose that serves as a valuable research tool for studying glucose metabolism and its impact on cellular pathways. By removing the hydroxyl group at the C-2 position and acetylating the remaining hydroxyls, this modified glucose analog prevents further phosphorylation, effectively inhibiting glycolysis. Researchers use this compound to understand the glycolytic flux in various biological systems by competing with glucose in cellular uptake and metabolism. The tetraacetate form allows for efficient cell penetration and rapid deacetylation, generating 2-deoxy-D-glucose within cells to act as a potent glycolysis inhibitor. Through these mechanisms, 2-Deoxy-D-glucose-tetraacetate has been instrumental in dissecting the intricacies of cellular glucose metabolism, providing insight into regulatory checkpoints of glycolysis and the hexosamine biosynthesis pathway. It has been used to study the metabolic dependencies of cancer cells, which often exhibit altered glucose metabolism, and to assess the effects of glucose depletion on immune cells and other highly glycolytic tissues. Furthermore, by impacting glycolysis, this compound allows researchers to probe the relationship between energy metabolism and cellular signaling pathways, revealing metabolic vulnerabilities and elucidating the regulation of various energy-sensitive processes.