2-Chloro-4-nitrophenyl-α-D-glucopyranoside (CAS 119047-14-2)

2-Chloro-4-nitrophenyl-α-D-glucopyranoside is a chemically synthesized glycoside that serves as a vital tool in biochemical research, particularly in the study of enzymatic activities involving glycosidases. This compound is designed to release a 2-chloro-4-nitrophenol moiety upon enzymatic cleavage by specific glycosidases, making it an excellent substrate for measuring enzyme kinetics and specificity. The chloro and nitro groups on the aromatic ring enhance the electron-withdrawing capacity, significantly affecting the rate of enzymatic cleavage, thereby providing insights into the mechanism of action of these enzymes. This glycoside helps in characterizing the activity of glycosidases under various conditions, contributing to a deeper understanding of their role in carbohydrate metabolism. The specificity of 2-Chloro-4-nitrophenyl-α-D-glucopyranoside for α-linkages in glucose polymers makes it particularly useful for studies related to the breakdown of complex carbohydrates, which is fundamental in cellular biology and biochemistry. This research tool enables scientists to probe the substrate preferences and kinetic behaviors of glycosidases, advancing our knowledge of these enzymes. These applications underscore the compound′s value in basic scientific inquiry and its role in enhancing our understanding of biochemical pathways and molecular interactions.