2,7-Di-tert-butyl-9,9-dimethylxanthene-4,5-dicarboxylic acid (CAS 130525-39-2)

2,7-Di-tert-butyl-9,9-dimethylxanthene-4,5-dicarboxylic acid, also known as 2,7-DI-Tert-butyl-9,9-dimethyl-4,5-xanthenedicarboxylic acid or DiTAD, is a fluorescent dye extensively utilized in scientific research due to its distinctive structure. This dye is employed to label proteins, lipids, and nucleic acids in cells and tissues, enabling researchers to track the movement of molecules in real-time through live-cell imaging studies. It has also proven valuable in flow cytometry and fluorescence microscopy, facilitating the detection and quantification of various cellular events.The fluorescent properties of 2,7-Di-tert-butyl-9,9-dimethylxanthene-4,5-dicarboxylic acid arise from its ability to absorb light at a specific wavelength and subsequently emit light at a longer wavelength, a process known as fluorescence. This phenomenon is attributed to the conjugation of the xanthene ring system with the carboxylic acid groups present in the molecule. As a result, 2,7-Di-tert-butyl-9,9-dimethylxanthene-4,5-dicarboxylic acid exhibits high fluorescence, emitting a vibrant red light.