2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (CAS 28752-68-3)

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) is a compound frequently employed in biochemical assays, particularly in the detection and quantification of enzymatic activity and antioxidant capacity. This chemical is renowned for its high sensitivity and wide applicability in various research fields, including biochemistry, molecular biology, and clinical diagnostics. ABTS is commonly utilized as a chromogenic substrate for peroxidase enzymes in assays such as ELISA (enzyme-linked immunosorbent assay) and HRP (horseradish peroxidase)-based immunoassays. In these assays, ABTS undergoes oxidation by the peroxidase enzyme in the presence of hydrogen peroxide, resulting in the formation of a green-colored product. The intensity of the color is directly proportional to the enzymatic activity, allowing for quantitative measurement using spectrophotometry. ABTS is frequently employed as a radical scavenger in antioxidant assays to assess the antioxidant capacity of natural compounds, food samples, and pharmaceutical formulations. Its ability to react with free radicals and transition metals, forming a stable blue-colored radical cation, makes it a valuable tool for evaluating the efficacy of antioxidants in neutralizing oxidative stress. In research, ABTS has also been utilized in kinetic studies, enzyme kinetics, and screening assays due to its rapid reaction kinetics and compatibility with high-throughput platforms. Furthermore, its stability in aqueous solutions and broad absorbance spectrum make it suitable for a wide range of experimental conditions. Ongoing research endeavors continue to explore novel applications and modifications of ABTS-based assays, aiming to enhance sensitivity, specificity, and versatility in biochemical research. Overall, 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) stands as a pivotal compound in biochemical research, facilitating the elucidation of enzymatic mechanisms, antioxidant properties, and molecular interactions critical to various biological processes.