11β-HSD2 Antibody (C-9): sc-365529

11β-HSD2 Antibody (C-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects 11β-HSD2 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). 11β-HSD2 (C-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. 11β-HSD2 plays a vital role in regulating glucocorticoid hormone action within target tissues by catalyzing the conversion of active corticosteroids, such as cortisol, into inactive forms, like cortisone. This enzymatic activity is particularly important in tissues where the balance of glucocorticoids and mineralocorticoids is critical for maintaining physiological homeostasis. 11β-HSD2 is predominantly expressed in the placenta and in aldosterone target tissues, including the kidney, parotid gland, colon, and skin, where 11β-HSD2 helps prevent inappropriate activation of mineralocorticoid receptors by cortisol. Dysregulation of 11β-HSD2 has been implicated in various conditions, including hypertension and metabolic disorders, highlighting 11β-HSD2′s significance in both development and disease. 11β-HSD2′s ability to modulate receptor occupancy by glucocorticoids underscores its potential as a therapeutic target in disorders related to glucocorticoid signaling.

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11β-HSD2 Antibody (C-9) References:

1. 11 beta-Hydroxysteroid dehydrogenase.  |  Stewart, PM. and Krozowski, ZS. 1999. Vitam Horm. 57: 249-324. PMID: 10232052
2. Expression of 11 beta-hydroxysteroid dehydrogenase in early pregnancy: implications in human trophoblast-endometrial interactions.  |  Arcuri, F., et al. 1999. Semin Reprod Endocrinol. 17: 53-61. PMID: 10406076
3. Functional expression, characterization, and purification of the catalytic domain of human 11-beta -hydroxysteroid dehydrogenase type 1.  |  Walker, EA., et al. 2001. J Biol Chem. 276: 21343-50. PMID: 11294832
4. Expression and putative role of 11 beta-hydroxysteroid dehydrogenase isozymes within the human eye.  |  Rauz, S., et al. 2001. Invest Ophthalmol Vis Sci. 42: 2037-42. PMID: 11481269
5. Improved lipid and lipoprotein profile, hepatic insulin sensitivity, and glucose tolerance in 11beta-hydroxysteroid dehydrogenase type 1 null mice.  |  Morton, NM., et al. 2001. J Biol Chem. 276: 41293-300. PMID: 11546766
6. The human gene for 11 beta-hydroxysteroid dehydrogenase. Structure, tissue distribution, and chromosomal localization.  |  Tannin, GM., et al. 1991. J Biol Chem. 266: 16653-8. PMID: 1885595
7. Foetal and placental 11β-HSD2: a hub for developmental programming.  |  Cottrell, EC., et al. 2014. Acta Physiol (Oxf). 210: 288-95. PMID: 24330050
8. Recent advances in the study of 11β-Hydroxysteroid dehydrogenase type 2 (11β-HSD2)Inhibitors.  |  Zhou, C., et al. 2017. Environ Toxicol Pharmacol. 52: 47-53. PMID: 28366868
9. Contribution of placental 11β-HSD2 to the pathogenesis of preeclampsia.  |  Wang, G., et al. 2020. FASEB J. 34: 15379-15399. PMID: 32978833
10. Cloning and tissue distribution of the human 11 beta-hydroxysteroid dehydrogenase type 2 enzyme.  |  Albiston, AL., et al. 1994. Mol Cell Endocrinol. 105: R11-7. PMID: 7859916
11. Cloning and production of antisera to human placental 11 beta-hydroxysteroid dehydrogenase type 2.  |  Brown, RW., et al. 1996. Biochem J. 313 (Pt 3): 1007-17. PMID: 8611140
12. 11 beta-hydroxysteroid dehydrogenase type II in human colon: a new marker of fetal development and differentiation in neoplasms.  |  Takahashi, K., et al. 1998. Anticancer Res. 18: 3381-8. PMID: 9858912

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-11β-HSD2 Antibody (C-9) has 19 citations in a variety of scientific publications.

Primary antibodies like Anti-11β-HSD2 Antibody (C-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

11β-HSD2 Antibody (C-9) Specifications

Alternate Product Name: HSD11B2

Product Clone Name: C-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 261-405

Epitope Target: 11β-HSD2

Epitope Species: human

Product Reactivity/Detection: 11β-HSD2

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 19 citations in various scientific publications

Available Conjugates/Forms for 11β-HSD2 Antibody (C-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin