1-β-D-Arabinofuranosyluracil-13C,15N2

1-β-D-Arabinofuranosyluracil-13C,15N2, a stable isotope-labeled derivative of ara-U, has become a valuable tool in scientific research, particularly in the field of nucleoside metabolism and nucleic acid labeling studies. This compound, synthesized by incorporating stable isotopes carbon-13 and nitrogen-15 into the molecular structure of ara-U, allows for precise tracking and quantification of nucleoside uptake, metabolism, and distribution in biological systems. Researchers utilize 1-β-D-Arabinofuranosyluracil-13C,15N2 as a tracer molecule to investigate various aspects of nucleoside metabolism, including biosynthesis, catabolism, and salvage pathways. By introducing isotopic labels into the nucleoside backbone, scientists can accurately trace the fate of ara-U in cellular and physiological processes, shedding light on its interactions with enzymes, transporters, and cellular targets. Moreover, this stable isotope-labeled compound serves as a valuable tool in nucleic acid research, enabling the synthesis of labeled nucleic acids for applications such as RNA sequencing, nucleic acid hybridization studies, and elucidating the mechanisms of action of antiviral and anticancer nucleoside analogs. The precise labeling pattern provided by 1-β-D-Arabinofuranosyluracil-13C,15N2 facilitates the differentiation of labeled nucleosides from endogenous counterparts, enabling accurate quantification and analysis of nucleic acid dynamics in biological systems.