1,2-Phenylene-bis-maleimide (CAS 13118-04-2)

1,2-Phenylene-bis-maleimide is a versatile chemical compound categorized as a maleimide. Within scientific research, it serves a role as both a crosslinking agent and a fluorescent probe. Its capability to form crosslinks between proteins and other biomolecules makes it indispensable in the fields of biochemistry and biophysics. As a crosslinking agent, 1,2-Phenylene-bis-maleimide finds widespread use in scientific research, specifically for proteins and other biomolecules. Additionally, it serves as a valuable fluorescent probe for studying the intricate structure and function of proteins. By employing 1,2-Phenylene-bis-maleimide, researchers have successfully crosslinked various biomolecules, including enzymes, antibodies, and DNA. Furthermore, it plays a role in the preparation of biomaterials such as hydrogels. The crosslinking process of 1,2-Phenylene-bis-maleimide involves the formation of stable covalent bonds between the compound and amino/thiol groups present in proteins. Notably, this crosslinking reaction is pH-dependent, with an optimal pH around 7.5. The fluorescence properties of 1,2-Phenylene-bis-maleimide originate from its ability to bind with proteins and other biomolecules, resulting in alterations in its fluorescence characteristics.