δ-catenin Double Nickase Plasmid (h): sc-402493-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- δ-catenin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- δ-catenin Double Nickase Plasmid (h) and δ-catenin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CTNND2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: δ-catenin Antibody (40.1): sc-81793
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
δ-catenin Double Nickase Plasmid (h) | sc-402493-NIC | 20 µg | $410.00 | |||
δ-catenin Double Nickase Plasmid (h2) | sc-402493-NIC-2 | 20 µg | $410.00 |
CTNND2 encodes human δ-catenin (p120-catenin family member), a cytoplasmic adaptor enriched at adherens junctions where it modulates cadherin–catenin complex stability and links cell–cell adhesion to actin cytoskeletal dynamics. δ-catenin participates in signaling pathways that regulate neurite outgrowth, synaptic organization, and cellular motility, in part through interactions with Rho family GTPase regulators and junctional scaffolding proteins. Altered CTNND2 expression or genomic disruption has been associated with neurodevelopmental phenotypes and has also been investigated in contexts of epithelial plasticity and tumor biology where adhesion remodeling is a key process. These features make CTNND2 a useful target for studying junctional signaling, neuronal morphogenesis, and mechanisms coupling adhesion to transcriptional and cytoskeletal programs.
δ-catenin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CTNND2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CTNND2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CTNND2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CTNND2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.