Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
β3 Tubulin Double Nickase Plasmid (h): sc-400525-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- β3 Tubulin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- β3 Tubulin Double Nickase Plasmid (h) and β3 Tubulin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TUBB3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: β3 Tubulin Antibody (2G10): sc-80005
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
β3 Tubulin Double Nickase Plasmid (h) | sc-400525-NIC | 20 µg | $410.00 | |||
β3 Tubulin Double Nickase Plasmid (h2) | sc-400525-NIC-2 | 20 µg | $410.00 |
TUBB3 encodes human β3 Tubulin, a neuron-enriched β-tubulin isotype that polymerizes with α-tubulin to form microtubules, supporting mitotic spindle dynamics, axonal growth, intracellular transport, and cytoskeletal remodeling. β3 Tubulin participates in microtubule-dependent processes that shape cell polarity and migration and influences vesicle trafficking through motor protein interactions. Altered TUBB3 expression or tubulin isotype composition has been linked to neurodevelopmental phenotypes and to cancer biology, where microtubule network regulation intersects with proliferation, invasion, and drug-response pathways. As a marker of neuronal differentiation and cytoskeletal state, TUBB3 is widely studied in models of neural lineage commitment and tumor cell plasticity.
β3 Tubulin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TUBB3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TUBB3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TUBB3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TUBB3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.