α1D-AR Double Nickase Plasmid (h): sc-402904-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- α1D-AR Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- α1D-AR Double Nickase Plasmid (h) and α1D-AR Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ADRA1D. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: α1D-AR Antibody (F-10): sc-390884
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
α1D-AR Double Nickase Plasmid (h) | sc-402904-NIC | 20 µg | $410.00 | |||
α1D-AR Double Nickase Plasmid (h2) | sc-402904-NIC-2 | 20 µg | $410.00 |
ADRA1D encodes the human α1D-adrenergic receptor (α1D-AR), a G protein–coupled receptor that preferentially signals through Gq/11 to activate phospholipase C, elevate intracellular Ca2+, and stimulate PKC-dependent transcriptional programs. α1D-AR contributes to smooth muscle contractility and vascular tone and modulates downstream MAPK/ERK signaling, ion channel activity, and Ca2+-dependent gene expression. Receptor activity integrates catecholamine inputs within autonomic signaling networks and can influence cellular proliferation and remodeling programs in responsive tissues. Dysregulated adrenergic signaling involving ADRA1D has been investigated in cardiovascular and urogenital physiology and in broader contexts where GPCR-driven Ca2+ signaling impacts inflammation and cell-state transitions.
α1D-AR Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ADRA1D locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ADRA1D. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ADRA1D function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ADRA1D-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.