α-sarcoglycan Double Nickase Plasmid (m): sc-422904-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- α-sarcoglycan Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- α-sarcoglycan Double Nickase Plasmid (m) and α-sarcoglycan Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Sgca. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: α-sarcoglycan Antibody (D-7): sc-271321
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
α-sarcoglycan Double Nickase Plasmid (m) | sc-422904-NIC | 20 µg | $410.00 |
Sgca encodes α-sarcoglycan, a core component of the sarcoglycan subcomplex within the dystrophin–glycoprotein complex that stabilizes the muscle fiber membrane and links the cytoskeleton to the extracellular matrix. In skeletal and cardiac muscle, α-sarcoglycan supports sarcolemmal integrity during contraction and contributes to mechanotransduction pathways that influence calcium handling, membrane repair, and cytoskeletal organization. Disruption of sarcoglycan-dependent architecture promotes membrane fragility, myofiber degeneration, and chronic remodeling, making Sgca a widely used locus for modeling dystrophin–glycoprotein complex dysfunction. Mouse Sgca perturbation is therefore relevant for studying muscle homeostasis, stress responses, and pathways associated with muscular dystrophy-like phenotypes.
α-sarcoglycan Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Sgca locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Sgca. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Sgca function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Sgca-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.