α3d Tubulin Double Nickase Plasmid (h): sc-400024-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- α3d Tubulin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- α3d Tubulin Double Nickase Plasmid (h) and α3d Tubulin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TUBA3D. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
α3d Tubulin Double Nickase Plasmid (h) | sc-400024-NIC | 20 µg | $410.00 | |||
α3d Tubulin Double Nickase Plasmid (h2) | sc-400024-NIC-2 | 20 µg | $410.00 |
TUBA3D encodes α3d tubulin, a β-tubulin–partnering structural subunit of microtubules that supports cytoskeletal organization, intracellular transport, and mitotic spindle assembly. As part of the tubulin heterodimer pool, α3d tubulin contributes to microtubule dynamic instability that underpins cell division, polarity, and vesicle trafficking, interfacing with pathways governed by microtubule-associated proteins and motor complexes such as kinesins and dynein. Perturbations in tubulin isotype expression and microtubule regulation are broadly relevant to aneuploidy, chromosomal instability, and altered cell motility, processes frequently studied in cancer biology and neurodevelopmental contexts. TUBA3D therefore serves as a useful target for dissecting microtubule-dependent phenotypes, cytoskeletal remodeling, and cell cycle progression in human cellular systems.
α3d Tubulin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TUBA3D locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TUBA3D. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TUBA3D function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TUBA3D-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.