α3C Tubulin Double Nickase Plasmid (h): sc-400020-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- α3C Tubulin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- α3C Tubulin Double Nickase Plasmid (h) and α3C Tubulin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TUBA3C. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
α3C Tubulin Double Nickase Plasmid (h) | sc-400020-NIC | 20 µg | $410.00 | |||
α3C Tubulin Double Nickase Plasmid (h2) | sc-400020-NIC-2 | 20 µg | $410.00 |
TUBA3C encodes human α3C tubulin, a core component of microtubules that supports cytoskeletal architecture, intracellular transport, and dynamic spindle formation during mitosis. Through regulated polymerization with other α/β-tubulin isoforms, α3C tubulin contributes to microtubule-dependent processes including cell polarity, vesicle trafficking, and chromosome segregation, integrating with pathways that control cell-cycle progression and cytoskeletal remodeling. Altered microtubule dynamics and tubulin isotype composition are frequently linked to genomic instability and dysregulated proliferation, making tubulin biology broadly relevant to studies of cancer cell behavior and neurodevelopmental cell functions. As part of the tubulin network, TUBA3C is also useful for dissecting how cytoskeletal perturbations affect organelle positioning, stress responses, and mitotic fidelity.
α3C Tubulin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TUBA3C locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TUBA3C. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TUBA3C function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TUBA3C-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.