Western Blot Stripping Buffer

사용법 :

• Wash already probed membrane with TBST (stock solutions available: sc-24953, sc-281695) for 5 min.

•Determine amount of stripping buffer needed (20 mL is usually sufficient for one membrane).

•Combine 20 mL of Buffer A and 200µL of Buffer B to make a 1:100 Buffer B: Buffer A dilution. For best results, let combined buffer come to room temperature prior to use.

•Incubate the membrane on an orbital shaker with sufficient stripping buffer to cover the membrane. Depending on the size and quantity, more than 20 mL may be needed.

•Incubate/shake for 30 min at RT. Results can be had in as little as 15 min, with up to 2 hrs for strongly bound antibodies.

•Wash 3x with TBST for 5 min.

•Reblock with appropriate blocking reagent, and perform western blot onto membrane according to Santa Cruz Biotechnology research applications or standard protocols.

공식화 :

Buffer A: Proprietary Component 1 40%, Water 60%
Buffer B: Proprietary component 2 10-20%, Water 80-90%

건강 상태 :

Liquid

저장 :

Store at 4° C