VAC14 항체 (F-12) WB, IP, IF, IHC(P)와 ELISA으로 mouse, rat와 human origin의 VAC14을 검출할것을 권장합니다.

VAC14 항체 (F-12): sc-365272

VAC14 항체 (F-12) is rated 5.0 out of 5 by 2.
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데이터 시트

    • VAC14 항체(F-12) 는 마우스 monoclonal IgG1(kappa light chain) 이며 200 µg/ml으로 제공합니다.
    • raised against amino acids 421-681 mapping within an internal region of VAC14 of human origin
    • 안티-VAC14 항체 (F-12)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 VAC14 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-VAC14 항체 (F-12)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • VAC14 (F-12): sc-365272무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP is the preferred secondary detection reagent for VAC14 Antibody (F-12) for WB and IHC(P) applications. This reagent is now offered in a bundle with VAC14 Antibody (F-12) (see ordering information below).
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Can VAC14 (F-12): sc-365272 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenniferc
Thank you for your question. Yes, VAC14 (F-12): sc-365272 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Great immunoperoxidase cytoplasmic and membrane Great immunoperoxidase cytoplasmic and membrane staining in formalin fixed, paraffin-embedded human gall bladder tissue. -SCBT QC
Date published: 2015-04-21
Rated 5 out of 5 by from Nice Western Blot data of VAC14 expression Nice Western Blot data of VAC14 expression in HeLa whole cell lysate. -SCBT QC
Date published: 2013-04-30
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