USP14 Antibody (C-5): sc-515812

USP14 Antibody (C-5) is a mouse monoclonal IgG2b antibody that detects USP14 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). USP14, also known as ubiquitin specific peptidase 14, is a crucial cytoplasmic protein that plays a significant role in the ubiquitin-proteasome system, which is essential for regulating protein degradation and maintaining cellular homeostasis. USP14 exists as a homodimer and functions primarily to remove ubiquitin moieties from ubiquitin-conjugated proteins, thereby preventing their degradation by the proteasome. USP14′s ability to rescue proteins from degradation is vital for various cellular processes, including signal transduction and transcriptional regulation, which are critical for normal cell function and development. Notably, defects in USP14 can lead to severe developmental issues, such as retarded growth or fetal death in mice, underscoring USP14′s importance in early developmental stages. Additionally, USP14 undergoes alternative splicing, resulting in multiple isoforms that may have distinct regulatory roles, further highlighting USP14′s complexity and significance in cellular biology. Anti-USP14 antibody (C-5) is an invaluable tool for researchers studying the intricacies of protein regulation and degradation pathways.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

USP14 Antibody (C-5) References:

1. USP14-mediated IκBα degradation exacerbates NF-κB activation and IL-1β-stimulated chondrocyte dedifferentiation.  |  Li, M., et al. 2019. Life Sci. 218: 147-152. PMID: 30550885
2. USP14 Inhibition Regulates Tumorigenesis by Inducing Autophagy in Lung Cancer In Vitro.  |  Han, KH., et al. 2019. Int J Mol Sci. 20: PMID: 31653087
3. USP14 regulates cell cycle progression through deubiquitinating CDK1 in breast cancer.  |  Liu, Y., et al. 2022. Acta Biochim Biophys Sin (Shanghai). 54: 1610-1618. PMID: 36604147
4. USP14 inhibition regulates tumorigenesis by inducing apoptosis in gastric cancer.  |  Lee, MY., et al. 2023. BMB Rep. 56: 451-456. PMID: 37401238
5. USP14 predicts poorer survival outcomes and promotes tumor progression in endometrial carcinoma by activating NF-κB signaling.  |  Gong, X., et al. 2023. Aging (Albany NY). 15: 12120-12135. PMID: 37917013
6. MitoCur-1 induces ferroptosis to reverse vemurafenib resistance in melanoma through inhibition of USP14.  |  Li, G., et al. 2024. Pigment Cell Melanoma Res. 37: 316-328. PMID: 37985430
7. GSTM3 enhances radiosensitivity of nasopharyngeal carcinoma by promoting radiation-induced ferroptosis through USP14/FASN axis and GPX4.  |  Chen, Y., et al. 2024. Br J Cancer. 130: 755-768. PMID: 38228715
8. USP14 promotes the cancer stem-like cell properties of OSCC via promoting SOX2 deubiquitination.  |  Liu, C., et al. 2024. Oral Dis. 30: 4255-4265. PMID: 38376172
9. Inhibition of Usp14 ameliorates renal ischemia-reperfusion injury by reducing Tfap2a stabilization and facilitating mitophagy.  |  Li, Y., et al. 2024. Transl Res. 270: 94-103. PMID: 38643868
10. USP14 modulates cell pyroptosis and ameliorates doxorubicin-induced cardiotoxicity by deubiquitinating and stabilizing SIRT3.  |  Zhang, Z., et al. 2024. Free Radic Biol Med. 225: 741-757. PMID: 39490774

The preferred USP14 antibody is USP14 Antibody (6E6): sc-100630.