TSPYL1 CRISPR/Cas9 KO Plasmid (m): sc-423529
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TSPYL1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TSPYL1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TSPYL1 CRISPR/Cas9 KO Plasmid (m) | sc-423529 | 20 µg | $397.00 |
Overview
Tspyl1 encodes TSPYL1, a nucleosome assembly protein (NAP) family member implicated in chromatin organization and transcriptional regulation. In mouse cells, TSPYL1 has been linked to control of cell-cycle progression and differentiation through epigenetic modulation of gene expression programs, influencing processes such as DNA-templated transcription and chromatin accessibility. By shaping chromatin states, TSPYL1 can affect pathways governing proliferation and cellular stress responses, making it relevant to studies of developmental phenotypes and oncogenic transformation mechanisms. Dysregulation of TSPYL family proteins has been associated with altered growth control and disease-relevant transcriptional signatures, supporting its use as a target in functional genomics.
TSPYL1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Tspyl1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Tspyl1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Tspyl1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TSPYL1 protein expression.
This CRISPR knockout system enables efficient generation of Tspyl1-deficient cell models for investigation of TSPYL1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Tspyl1 exon(s) critical for TSPYL1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Tspyl1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TSPYL1 CRISPR/Cas9 KO Plasmid (m) and TSPYL1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Tspyl1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TSPYL1 HDR Plasmid (m) and TSPYL1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Tspyl1 homology arms to support homology-directed repair at defined Tspyl1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.