Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TRPC1 Double Nickase Plasmid (m): sc-423512-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TRPC1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TRPC1 Double Nickase Plasmid (m) and TRPC1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Trpc1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TRPC1 Antibody (E-6): sc-133076
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TRPC1 Double Nickase Plasmid (m) | sc-423512-NIC | 20 µg | $410.00 | |||
TRPC1 Double Nickase Plasmid (m2) | sc-423512-NIC-2 | 20 µg | $410.00 |
Trpc1 encodes TRPC1, a nonselective cation channel subunit that contributes to receptor-operated and store-operated Ca2+ entry in many mouse cell types. By shaping intracellular Ca2+ dynamics, TRPC1 modulates processes including membrane excitability, cytoskeletal remodeling, cell migration, and transcriptional programs downstream of PLC-coupled receptors and STIM–ORAI signaling. TRPC1 activity intersects with MAPK and NFAT-dependent pathways, influencing cellular differentiation and stress responses. Dysregulated TRPC1-mediated Ca2+ homeostasis has been implicated in experimental models of cardiovascular remodeling, neuroinflammation, and fibrotic signaling, supporting its relevance for mechanistic studies of disease-associated pathways.
TRPC1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Trpc1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Trpc1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Trpc1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Trpc1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.