TRIM34 CRISPR/Cas9 KO Plasmid (h): sc-417920
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TRIM34 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TRIM34 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TRIM34 CRISPR/Cas9 KO Plasmid (h) | sc-417920 | 20 µg | $397.00 |
Overview
TRIM34 encodes a tripartite motif-containing E3 ubiquitin ligase of the TRIM family implicated in innate immune regulation and antiviral restriction. Through its RING, B-box, and coiled-coil domains, TRIM34 can influence ubiquitin-dependent signaling and protein turnover, shaping pathways such as interferon-stimulated responses and pattern-recognition receptor signaling. Altered TRIM family activity is frequently linked to dysregulated inflammation and host–pathogen interactions, making TRIM34 relevant for studying mechanisms that modulate immune homeostasis. TRIM34 is also used as a candidate regulator in systems-level analyses of infection biology and immune-associated disease phenotypes.
TRIM34 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TRIM34 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TRIM34 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TRIM34 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TRIM34 protein expression.
This CRISPR knockout system enables efficient generation of TRIM34-deficient cell models for investigation of TRIM34 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TRIM34 exon(s) critical for TRIM34 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TRIM34 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TRIM34 CRISPR/Cas9 KO Plasmid (h) and TRIM34 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TRIM34 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TRIM34 HDR Plasmid (h) and TRIM34 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TRIM34 homology arms to support homology-directed repair at defined TRIM34 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.