human origin의 TREX-2에서 C-terminus에 위치한 248-268의 아미노산사이에 위치한 항원결정부와 특정결합합니다.
안티-TREX-2 항체 (E-4)는 WB, IP, IF and ELISA으로 mouse, rat and human유래의 TREX-2 를 감지하는 데에 추천한다.
IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-TREX-2 항체 (E-4)를 제공한다.
WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
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TREX-2 Antibody (E-4) is a high quality monoclonal TREX-2 antibody (also designated TREX-2 antibody) suitable for the detection of the TREX-2 protein of mouse, rat and human origin. TREX-2 Antibody (E-4) is available as both the non-conjugated anti-TREX-2 antibody form, as well as multiple conjugated forms of anti-TREX-2 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Proper DNA and RNA metabolism requires nucleases which function in DNA replication, recombination and repair, as well as in RNA processing and degradation events. TREX-2 (three prime repair exonuclease 2) is a 279 amino acid protein that localizes to the nucleus and belongs to the TREX family of exonuclease proteins. Expressed in stomach, heart, breast, testis, prostate, colon and skeletal muscle, TREX-2 exists as a homodimer that functions as an exonuclease and is thought to play a role in DNA repair. Specifically, TREX-2 uses magnesium to catalyze the 3′ to 5′ exonucleolytic cleavage of 3′ mismatched double stranded DNA, thereby yielding nucleoside 5′ phosphates and eliminating mutant DNA. Multiple isoforms of TREX-2 exists due to alternative splicing events. Of transcriptional interest, the gene encoding human TREX-2 (Xq28) is either identical to or adjacent to that of UCHL5IP as most mRNAs encoding UCHL5IP also include the N-terminal part of TREX2.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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What application is the blocking peptide sc-390890 P appropriate for?
Asked by: Randy McDonald
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here:
Rated 4 out of
Works well for IPUsed this for IP of HCT 116 human colorectal carcinoma.
Cells lysed in RIPA, 30 min @ 4 C rocking, then pelleted and sup collected/OD
Mixed 500 ug total lysate + 3 ug ab, end-over-end rocking 1 h @ 4 C
Added 20 ul Protein A/G Bead slurry (sc-2003), rocked 2 h more @ 4 C
(Collected / pelleted resin at 1,000 x g for 5 min for all washes)
Washed 3 x 5 min in RIPA, then 1 x 5 min in PBS (both @ 4 C)
Resuspended beads in equal volume 2x SDS loading dye (~15 ul) and boiled 5 min
TREX2 (E4) Ab 1:400 2 h @ RT
anti-mouse IgG HRP 1:12,000 1 h @ RT
Washed 3 x 10 min in TBS-T after each ab incubation
Developed in ECL substrate 10 min before exposing to film. 1 min exposure shown below.
Signal was robust so next time, I think I can easily decrease the amount of protein used for IP and/or increase the ab dilution to 1:500 or higher.
Date published: 2017-06-14
Rated 5 out of
Produced positive Western blot data of TREXProduced positive Western blot data of TREX-2 expression in CCRF-CEM and MOLT-4 nuclear extracts and mouse liver and human liver tissue extracts. -SCBT QC