TREX-2 항체 (E-4) WB, IP, IF와 ELISA으로 mouse, rat와 human origin의 TREX-2을 검출할것을 권장합니다.

TREX-2 항체 (E-4): sc-390890

TREX-2 항체 (E-4) is rated 4.5 out of 5 by 2.
  • y_2020, m_8, d_5, h_16
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.10
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_ko_KR, sid_390890, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 332ms
  • REVIEWS, PRODUCT
5
1
4
1
3
0
2
0
1
0

Datasheets

    • 안티-TREX-2 항체 E-4 는 마우스 monoclonal IgG1 (kappa light chain) TREX-2 항체 이며 200 µg/ml으로 제공합니다.
    • human origin의 TREX-2에서 C-terminus에 위치한 248-268의 아미노산사이에 위치한 항원결정부와 특정결합합니다.
    • 안티-TREX-2 항체 (E-4)는 WB, IP, IF and ELISA으로 mouse, rat and human유래의 TREX-2 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-TREX-2 항체 (E-4)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • blocking peptide, sc-390890 P
    • 강력추천하는 conventional secondary anti-mouse IgG reagents의 대체제품 보기m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP).
    • TREX-2 (E-4): sc-390890무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
이 제품의 리뷰를 쓰고 15 CruzCredits를 받기

What application is the blocking peptide sc-390890 P appropriate for?

Asked by: Randy McDonald
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-25
  • y_2020, m_8, d_5, h_16CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.10
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_ko_KR, sid_390890, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 144ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from Works well for IP Used this for IP of HCT 116 human colorectal carcinoma. Cells lysed in RIPA, 30 min @ 4 C rocking, then pelleted and sup collected/OD IP: Mixed 500 ug total lysate + 3 ug ab, end-over-end rocking 1 h @ 4 C Added 20 ul Protein A/G Bead slurry (sc-2003), rocked 2 h more @ 4 C (Collected / pelleted resin at 1,000 x g for 5 min for all washes) Washed 3 x 5 min in RIPA, then 1 x 5 min in PBS (both @ 4 C) Resuspended beads in equal volume 2x SDS loading dye (~15 ul) and boiled 5 min WB: TREX2 (E4) Ab 1:400 2 h @ RT anti-mouse IgG HRP 1:12,000 1 h @ RT Washed 3 x 10 min in TBS-T after each ab incubation Developed in ECL substrate 10 min before exposing to film. 1 min exposure shown below. Signal was robust so next time, I think I can easily decrease the amount of protein used for IP and/or increase the ab dilution to 1:500 or higher.
Date published: 2017-06-14
Rated 5 out of 5 by from Produced positive Western blot data of TREX Produced positive Western blot data of TREX-2 expression in CCRF-CEM and MOLT-4 nuclear extracts and mouse liver and human liver tissue extracts. -SCBT QC
Date published: 2013-06-12
  • y_2020, m_8, d_5, h_16
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.10
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_ko_KR, sid_390890, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 18ms
  • REVIEWS, PRODUCT

산타크루즈바이오테크놀러지는 생물의학연구에 필요한 제품을 개발하는 선구자입니다. Toll Free 1-800-457-3801로 연락주세요. Copyright © 2007-2020 Santa Cruz Biotechnology, Inc. 판권소유. "Santa Cruz Biotechnology", 와 Santa Cruz Biotechnology, Inc. 로고, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", 및 San Juan Ranch 로고, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz"와 "EZ Touch" 는 Santa Cruz Biotechnology, Inc의 등록된 상표입니다. 모든 상표는 각자 소유주의 소유입니다.