TMPRSS11A Double Nickase Plasmid (h): sc-415530-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TMPRSS11A Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TMPRSS11A Double Nickase Plasmid (h) and TMPRSS11A Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TMPRSS11A. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TMPRSS11A Double Nickase Plasmid (h) | sc-415530-NIC | 20 µg | $410.00 |
TMPRSS11A encodes a type II transmembrane serine protease expressed at epithelial surfaces, where it contributes to pericellular proteolysis and protease-dependent remodeling of the extracellular milieu. Through regulated cleavage of protein substrates at the cell membrane, TMPRSS11A can influence epithelial barrier homeostasis, differentiation programs, and inflammatory signaling cascades linked to mucosal responses. Altered expression of TMPRSS11A has been reported in airway and gastrointestinal epithelia and has been explored in the context of epithelial stress states and tumor-associated protease networks. These properties make TMPRSS11A a useful target for investigating membrane-tethered protease function, proteolytic signaling, and epithelial biology.
TMPRSS11A Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TMPRSS11A locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TMPRSS11A. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TMPRSS11A function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TMPRSS11A-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.