TLR9 Antibody (E-9): sc-518138
- TLR9 Antibody (E-9) is a mouse monoclonal IgM κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 1012-1031 of TLR9 of mouse origin
- recommended for detection of TLR9 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-TLR9 Antibody (E-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for TLR9 Antibody (E-9) for WB applications. This reagent is now offered in a bundle with TLR9 Antibody (E-9) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
TLR9 Antibody (E-9) is a mouse monoclonal IgM antibody that detects TLR9 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TLR9 (E-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TLR9, a member of the Toll-like receptor family, plays a crucial role in the innate immune system by recognizing specific pathogen-associated molecular patterns, particularly unmethylated CpG DNA found in bacterial and viral genomes. This recognition triggers a cascade of immune responses, including the activation of NFκB signaling pathways, which are vital for the production of pro-inflammatory cytokines and the subsequent orchestration of adaptive immunity. The strategic expression of TLR9 in immune-rich tissues such as the spleen, lymph nodes, and bone marrow underscores its importance in the early detection of infections and the initiation of immune responses. Anti-TLR9 antibody (E-9) enables researchers to gain insights into the mechanisms of immune activation and the role of TLR9 in various diseases, including autoimmune disorders and infections.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TLR9 Antibody (E-9) References:
- Development of TLR9 agonists for cancer therapy. | Krieg, AM. 2007. J Clin Invest. 117: 1184-94. PMID: 17476348
- Toll-like receptor 9 (TLR9) agonists in the treatment of cancer. | Krieg, AM. 2008. Oncogene. 27: 161-7. PMID: 18176597
- TLR9 regulates adipose tissue inflammation and obesity-related metabolic disorders. | Hong, CP., et al. 2015. Obesity (Silver Spring). 23: 2199-206. PMID: 26260403
- IRAP+ endosomes restrict TLR9 activation and signaling. | Babdor, J., et al. 2017. Nat Immunol. 18: 509-518. PMID: 28319098
- TLR9 signaling in fibroblastic reticular cells regulates peritoneal immunity. | Xu, L., et al. 2019. J Clin Invest. 129: 3657-3669. PMID: 31380807
- B cell-intrinsic TLR9 expression is protective in murine lupus. | Tilstra, JS., et al. 2020. J Clin Invest. 130: 3172-3187. PMID: 32191633
- Insights into innate immune activation via PS-ASO-protein-TLR9 interactions. | Pollak, AJ., et al. 2022. Nucleic Acids Res. 50: 8107-8126. PMID: 35848907
- Ibrutinib sensitizes CLL cells to venetoclax by interrupting TLR9-induced CD40 upregulation and protein translation. | Kielbassa, K., et al. 2023. Leukemia. 37: 1268-1276. PMID: 37100883
- TLR9 signalling activation via direct ligation and its functional consequences in CD4 + T cells. | Sharma, RK., et al. 2022. Scand J Immunol. 96: PMID: 37406035
- IFNγ initiates TLR9-dependent autoimmune hepatitis in DNase II deficient mice. | Hao, K., et al. 2024. bioRxiv.. PMID: 39071327
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TLR9 Antibody (E-9) | sc-518138 | 200 µg/ml | $322.00 | |||
TLR9 Antibody (E-9): m-IgGκ BP-HRP Bundle | sc-551129 | 200 µg Ab; 40 µg BP | $361.00 | |||
TLR9 Antibody (E-9) AC | sc-518138 AC | 500 µg/ml, 25% agarose | $424.00 | |||
TLR9 Antibody (E-9) HRP | sc-518138 HRP | 200 µg/ml | $322.00 | |||
TLR9 Antibody (E-9) FITC | sc-518138 FITC | 200 µg/ml | $336.00 | |||
TLR9 Antibody (E-9) PE | sc-518138 PE | 200 µg/ml | $349.00 | |||
TLR9 Antibody (E-9) Alexa Fluor® 488 | sc-518138 AF488 | 200 µg/ml | $364.00 | |||
TLR9 Antibody (E-9) Alexa Fluor® 546 | sc-518138 AF546 | 200 µg/ml | $364.00 | |||
TLR9 Antibody (E-9) Alexa Fluor® 594 | sc-518138 AF594 | 200 µg/ml | $364.00 | |||
TLR9 Antibody (E-9) Alexa Fluor® 647 | sc-518138 AF647 | 200 µg/ml | $364.00 | |||
TLR9 Antibody (E-9) Alexa Fluor® 680 | sc-518138 AF680 | 200 µg/ml | $364.00 | |||
TLR9 Antibody (E-9) Alexa Fluor® 790 | sc-518138 AF790 | 200 µg/ml | $364.00 |