TBS Blotto A 일차 항체하고 이차 항체의 비특이적인 결합과 배경을 줄이기 위한 Blocking buffer 하고 antibody diluent .BSA보다 효과 더 좋다.

TBS Blotto A: sc-2333

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데이터 시트

    • TBS Blotto A is supplied as a lyophilized powder in a single-use bottle; final reconstituted volume of 500 mL
    • 일차 항체하고 이차 항체의 비특이적인 결합과 배경을 줄이기 위한 Blocking buffer 하고 antibody diluent .BSA보다 효과 더 좋다.
    • contains 5% milk, 0.05% Tween-20; Tween-20 is included in a separate vial in liquid form
    • deionized H2O 로 죄종500 ml 1x solution 다시 구성된다
    • TBS Blotto A is the preferred choice of blocking buffer and antibody diluent over BSA or alternative blocking buffers; strongly recommended to be used as an antibody diluent for both primary and secondary antibody incubations to reduce non-specific binding and background
    • HRP conjugated 일차 항체하고 이차 항체, 그니고 mouse IgG binding proteins 이용하고 enhanced chemiluminescent (ECL) protein detection methods 할 때 모든 배경을 줄이고 비특이적인 산호를 최소화하는 데에 추천합나다.
    • 직접 결합 일차 항체나 near-infrared (NIR) detection methods에 대해서는 UltraCruz® Blocking Reagent (sc-516214)강력히 추천합니다.
    • For phospho-specific antibodies, we recommend our alternative product, TBS Blotto B (sc-2335) which contains a unique formulation of 1% milk, 1% blocking reagent, 0.05% Tween-20
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