T-bet/TBX21 Antibody (D-5): sc-137114

T-bet Antibody (D-5) is a mouse monoclonal IgG2b antibody that detects T-bet in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). T-bet, a T box transcription factor, plays a crucial role in the differentiation of T helper cells, specifically promoting the Th1 subset while inhibiting Th2 development. This regulation is vital for the immune response, as Th1 cells are essential for combating intracellular pathogens and driving cell-mediated immunity. T-bet (D-5) antibody recognizes T-bet that is selectively expressed in Th1 cells, and T-bet expression is upregulated by signals from the T cell receptor and interleukin-12, which are critical for the activation and function of these immune cells. The ability of T-bet to convert effector Th2 cells into Th1 cells highlights its importance in maintaining the balance between these subsets, which is crucial for a well-regulated immune response. Anti-T-bet antibody (D-5) was originally isolated from nuclear extracts of resting and PMA/ionomycin-activated AE7 cells, demonstrating specificity and effectiveness in detecting T-bet levels in various experimental conditions.

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T-bet/TBX21 Antibody (D-5) References:

1. T-bet and Eomesodermin in NK Cell Development, Maturation, and Function.  |  Simonetta, F., et al. 2016. Front Immunol. 7: 241. PMID: 27379101
2. T-bet and Eomes govern differentiation and function of mouse and human NK cells and ILC1.  |  Zhang, J., et al. 2018. Eur J Immunol. 48: 738-750. PMID: 29424438
3. CD8+T-bet+ cells as a predominant biomarker for inclusion body myositis.  |  Dzangué-Tchoupou, G., et al. 2019. Autoimmun Rev. 18: 325-333. PMID: 30825520
4. Human T-bet Governs Innate and Innate-like Adaptive IFN-γ Immunity against Mycobacteria.  |  Yang, R., et al. 2020. Cell. 183: 1826-1847.e31. PMID: 33296702
5. Human T-bet governs the generation of a distinct subset of CD11chighCD21low B cells.  |  Yang, R., et al. 2022. Sci Immunol. 7: eabq3277. PMID: 35867801
6. T-bet+ B cells promote metabolic disease.  |  Tysoe, O. 2022. Nat Rev Endocrinol. 18: 589. PMID: 35970892
7. Early T-bet promotes LFA1 upregulation required for CD8+ effector and memory T cell development.  |  Pritchard, GH., et al. 2023. J Exp Med. 220: PMID: 36445307
8. Vaccine adjuvant-elicited CD8+ T cell immunity is co-dependent on T-bet and FOXO1.  |  Ivanova, DL., et al. 2023. Cell Rep. 42: 112911. PMID: 37516968
9. Differential Runx3, Eomes, and T-bet expression subdivides MS-associated CD4+ T cells with brain-homing capacity.  |  Hoeks, C., et al. 2024. Eur J Immunol. 54: e2350544. PMID: 38009648
10. Plasticity and lineage commitment of individual TH1 cells are determined by stable T-bet expression quantities.  |  Hegazy, AN., et al. 2024. Sci Adv. 10: eadk2693. PMID: 38838155

The preferred T-bet/TBX21 antibody is T-bet/TBX21 Antibody (4B10): sc-21749.