Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Synaptotagmin IV CRISPR/Cas9 KO Plasmid (m): sc-423246
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Synaptotagmin IV CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Synaptotagmin IV genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Synaptotagmin IV Antibody (H-4): sc-271936
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Synaptotagmin IV CRISPR/Cas9 KO Plasmid (m) | sc-423246 | 20 µg | $397.00 |
Overview
Syt4 encodes synaptotagmin IV, an atypical synaptotagmin family member implicated in activity-dependent regulation of vesicle trafficking and exocytosis in neurons and neuroendocrine cells. Unlike canonical Ca2+ sensors, synaptotagmin IV modulates synaptic vesicle cycling, neurotransmitter release probability, and presynaptic plasticity, linking membrane dynamics to neuronal activity programs. Syt4 function intersects with SNARE-mediated fusion machinery, endo/exocytic membrane recycling, and calcium-dependent signaling pathways that shape circuit-level adaptation. Dysregulated SYT4 expression or function has been associated with altered synaptic plasticity phenotypes and is studied in the context of neurodevelopmental and neuropsychiatric disease-relevant mechanisms.
Synaptotagmin IV CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Syt4 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Syt4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Syt4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Synaptotagmin IV protein expression.
This CRISPR knockout system enables efficient generation of Syt4-deficient cell models for investigation of Synaptotagmin IV signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Syt4 exon(s) critical for Synaptotagmin IV function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Syt4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Synaptotagmin IV CRISPR/Cas9 KO Plasmid (m) and Synaptotagmin IV CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Syt4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Synaptotagmin IV HDR Plasmid (m) and Synaptotagmin IV HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Syt4 homology arms to support homology-directed repair at defined Syt4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.