Syk 항체 (4D10) WB, IP, IF, IHC(P)와 FCM으로 mouse, rat와 human origin의 Syk을 검출할것을 권장합니다.

Syk 항체 (4D10): sc-1240

Syk 항체 (4D10) is rated 4.7 out of 5 by 17.
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Datasheets

    • 안티-Syk 항체 4D10 는 마우스 monoclonal IgG2a (kappa light chain) Syk 항체 이며 200 µg/ml으로 제공합니다.
    • human origin의 Syk에서 내부에 위치한 313-339 아미노산을 항원으로 사용하였습니다.
    • 안티-Syk 항체 (4D10)는 WB, IP, IF, IHC(P) and FCM으로 mouse, rat and human유래의 Syk 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-Syk 항체 (4D10)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • 강력추천하는 conventional secondary anti-mouse IgG reagents의 대체제품 보기m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP).
    • Syk (4D10): sc-1240무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
이 제품의 리뷰를 쓰고 15 CruzCredits를 받기

How to grade the positivity in breast carcinoma cells

Asked by: Ghazala
Thank you for your question. You would require appropriate positive and negative controls to compare the signals between different samples. Recommended positive controls are NAMALWA cell lysate: sc-2234, BJAB whole cell lysate: sc-2207 or Raji whole cell lysate: sc-364236. We also recommend using direct conjugates for detection so that signal strength directly corresponds to the amount of protein present.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

Is it possible for the Syk Antibody (4D10): sc-1240 to react with monkey tissue samples?

Asked by: Trav11
To inquire about additional species reactivity not listed on the webpage, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function. We will be more than happy to run sequence analysis for your species of interest.
Answered by: Tech Service 8
Date published: 2016-12-22
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Rated 4 out of 5 by from Works good! Good a free sample to test. Planing to use in future! For WB perferct!
Date published: 2018-02-13
Rated 5 out of 5 by from Great antibody for western blot The batch of antibody I have worked well on HeLa, NIH/3T3, Jurkat and K562 total cell extracts for western Blot. The band is specific. The antibody was diluted 500 times into 5% BSA, into TBST and incubated over-night at 4°C with the membrane.
Date published: 2017-11-16
Rated 5 out of 5 by from The antibody worked in western blot This antibody worked very well in western blot on mouse osteoclasts extract. We diluted it at 1/200 but it could be diluted more because the signal was strong.
Date published: 2017-10-25
Rated 5 out of 5 by from It works great. I tested in PC3 cells. It worked well at 1:200 dilution.
Date published: 2017-09-27
Rated 5 out of 5 by from worked well. I got this antibody as free samples and it worked very well for WB. I used 1:500 dilution.
Date published: 2017-03-15
Rated 5 out of 5 by from Excellent antibody for westerns Probed this antibody at 1/1000 dilution for one hour and used the Kappa-binding HRP as secondary: the result was flawless. Excellent antibody for westerns.
Date published: 2017-03-08
Rated 5 out of 5 by from Ottima reattività Utilizzato per rilevare la chinasi Syk in cellule Natural Killer primarie. Ottima reattività sia su whole cell lysates che in immunoprecipitazione.
Date published: 2017-02-06
Rated 5 out of 5 by from Well published Many citations using this antibody in a variety of applications, especially Western Blot and IP!
Date published: 2016-12-27
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