SV40 T Ag Antibody (Pab 430): sc-53449
- SV40 T Ag Antibody (Pab 430) is a mouse monoclonal IgG3 κ provided at 200 µg/ml
- raised against SV40 transformed cells of SV40 origin
- recommended for detection of large T antigen of SV40 origin by WB and IP
- See SV40 T Ag (Pab 101): sc-147 for SV40 T Ag antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG3 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for SV40 T Ag Antibody (Pab 430) for WB applications. These reagents are now offered in bundles with SV40 T Ag Antibody (Pab 430) (see ordering information below).
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SV40 T Ag Antibody (Pab 430) is a mouse monoclonal IgG3 antibody that detects SV40 T Ag in SV40 by western blotting (WB) and immunoprecipitation (IP). SV40 T Ag plays a crucial role in the viral life cycle and is essential for the transformation of host cells, as SV40 T Ag interacts with key cellular proteins that regulate cell growth and division. Notably, SV40 T Ag binds to the retinoblastoma (Rb) protein, specifically targeting the "pocket" domain, which is also the binding site for the E2F transcription factor. This interaction leads to the inactivation of Rb, disrupting Rb′s ability to regulate the cell cycle and promoting uncontrolled cell proliferation. Additionally, SV40 T Ag′s ability to form complexes with other tumor suppressor proteins, such as p53, further underscores SV40 T Ag′s importance in oncogenic processes. Anti-SV40 T Ag antibody (Pab 430) serves as an invaluable tool for researchers studying the mechanisms of viral oncogenesis and the intricate pathways involved in cellular transformation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SV40 T Ag Antibody (Pab 430) References:
- Phenotypic conversion of SV40-immortalized human diploid fibroblasts to senescing cells by introduction of an antisense gene for SV40-T antigen. | Tanaka, Y., et al. 1992. Cell Struct Funct. 17: 351-62. PMID: 1338304
- Multistep hepatocarcinogenesis in transgenic mice harboring SV40 T-antigen gene. | Kitagawa, T., et al. 1991. Princess Takamatsu Symp. 22: 349-60. PMID: 1668891
- Expression of SV40 T antigen during the cell cycle of sv40-transformed cells. | Stenman, S., et al. 1975. Int J Cancer. 15: 547-54. PMID: 166940
- SV40 T antigen binds directly to the large subunit of purified DNA polymerase alpha. | Dornreiter, I., et al. 1990. EMBO J. 9: 3329-36. PMID: 1698613
- Expression of SV40 T antigen under control of rabbit uteroglobin promoter in transgenic mice. | DeMayo, FJ., et al. 1991. Am J Physiol. 261: L70-6. PMID: 1872417
- Embryonic retinal tumors in SV40 T-Ag transgenic mice contain CD133+ tumor-initiating cells. | Wadhwa, L., et al. 2012. Invest Ophthalmol Vis Sci. 53: 3454-62. PMID: 22562503
- The piggyBac transposon-mediated expression of SV40 T antigen efficiently immortalizes mouse embryonic fibroblasts (MEFs). | Wang, N., et al. 2014. PLoS One. 9: e97316. PMID: 24845466
- SV40 T-antigen is a histocompatibility antigen of SV40-transgenic mice. | Wettstein, PJ., et al. 1988. Immunogenetics. 27: 436-41. PMID: 2836306
- c-myc protein can be substituted for SV40 T antigen in SV40 DNA replication. | Iguchi-Ariga, SM., et al. 1987. Nucleic Acids Res. 15: 4889-99. PMID: 3037484
- The C3(1)/SV40 T antigen transgenic mouse model of prostate and mammary cancer. | Shibata, MA., et al. 1998. Toxicol Pathol. 26: 177-82. PMID: 9502400
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SV40 T Ag Antibody (Pab 430) | sc-53449 | 200 µg/ml | $322.00 | |||
SV40 T Ag Antibody (Pab 430): m-IgGκ BP-HRP Bundle | sc-553206 | 200 µg Ab; 40 µg BP | $361.00 | |||
SV40 T Ag Antibody (Pab 430): m-IgG3 BP-HRP Bundle | sc-553207 | 200 µg Ab; 40 µg BP | $361.00 |