ST2 Double Nickase Plasmid (h): sc-402390-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ST2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ST2 Double Nickase Plasmid (h) and ST2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting IL1RL1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ST2 Antibody (14J07): sc-74296
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ST2 Double Nickase Plasmid (h) | sc-402390-NIC | 20 µg | $410.00 | |||
ST2 Double Nickase Plasmid (h2) | sc-402390-NIC-2 | 20 µg | $410.00 |
Human IL1RL1 encodes ST2, an interleukin-1 receptor family member that serves as the cognate receptor for IL-33 and modulates innate and adaptive immune signaling. ST2 exists as membrane-bound and soluble isoforms that shape IL-33–dependent pathways, including MyD88-dependent activation of NF-κB and MAPK signaling, influencing cytokine production, tissue remodeling, and barrier inflammation. IL1RL1/ST2 activity is closely linked to type 2 immune responses in epithelial and stromal compartments and is frequently studied in the context of allergic inflammation and asthma-associated signaling networks. Dysregulated IL-33–ST2 axis function is also investigated in cardiovascular and fibrotic processes where immune–stromal crosstalk contributes to chronic inflammation.
ST2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the IL1RL1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within IL1RL1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt IL1RL1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of IL1RL1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.