Date published: 2025-9-6
Santa Cruz 동물 건강 방문하기

001 800-1338-3838

SCBT Portrait Logo
Seach Input
연락하기
사인/가입 계정 번호
빠른 주문
장바구니 Items (0)
Sox9 Antibody (E-9) - Western Blotting - Image 384304
Sox-9 항체(E-9) HRP: sc-166505 HRP. 비감염된 Sox-9 발현의 직접 웨스턴 블롯 분석: sc-117752(A), 감염된 인간 Sox-9: sc-126032(B) 전체 세포 용해물 및 SW480 핵 추출물(C).
Sox-9 항체(E-9) HRP: sc-166505 HRP. 비감염된 Sox-9 발현의 직접 웨스턴 블롯 분석: sc-117752(A), 감염된 인간 Sox-9: sc-126032(B) 전체 세포 용해물 및 SW480 핵 추출물(C).

Sox9 항체 (E-9): sc-166505

4.1(34)
리뷰 쓰기질문하기

데이터 시트
  • Sox9 항체 E-9 는 마우스 monoclonal IgG2a κ Sox9 항체, 79간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • human origin의 Sox-9에서 N-terminus에 위치한 36-55의 아미노산사이에 위치한 항원결정부와 특정결합합니다.
  • Sox9 항체 (E-9)는 WB, IP, IF and ELISA으로mouse, rat, human and avian유래의 Sox-9 를 감지하는 데에 추천한다 ; 이외에, equine, canine, bovine, porcine and avian등 species와 반응할수 있습니다
  • 항-Sox9 항체(E-9)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • Gel Supershift와 ChIP 에 사용하는 TransCruz시약(sc-166505 X, 200 µg/0.1 ml)이 있습니다.
  • Sox9 (E-9): sc-166505의 무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRP, 2a BP-HRP">m-IgG2a BP-HRP 및 m-IgGκ BP-HRP는 Sox9 항체(E-9) WB 애플리케이션용입니다. 이 시약은 이제 Sox9 항체(E-9)와 함께 번들로 제공됩니다(아래 주문 정보 참조).

빠른 링크

보조 제품
설명
Gene 정보
데이터 시트 및 프로토콜
연구 정보

더보기

추가 Sox Antibodies 포함 Sox-10, Sox-13, Sox-15, Sox-17, Sox-18, Sox-2, Sox-21, Sox-22, Sox-3, Sox-30, Sox-4, Sox-5, Sox-6, Sox-8 및 Sox-9

Sox-9 항체(E-9)는 마우스, 쥐 및 인간 유래의 Sox-9 단백질을 WB, IP, IF 및 ELISA로 검출하는 IgG2a κ 마우스 단일 클론 Sox-9 항체(SOX9 항체 또는 SRY 관련 HMG-box 전사인자 9 항체로도 지정됨)입니다. Sox-9 항체(E-9)는 비접합 항-Sox-9 항체 형태뿐만 아니라 아가로스, HRP, PE, FITC 및 여러 Alexa Fluor® 접합체를 포함한 여러 접합 형태의 항-Sox-9 항체 형태로도 사용할 수 있습니다. Sox 유전자는 포유류의 성 결정 유전자 SRY와 관련된 유전자 계열로 구성되어 있습니다. 이 유전자들은 유사하게 서열 특이적 DNA 결합 활동을 담당하는 HMG-박스 도메인을 코딩하는 서열을 포함하고 있습니다. Sox 유전자는 발달 중 세포의 운명 결정과 다양한 발달 과정의 제어에 관여하는 전사 조절 인자를 암호화합니다. 매우 복잡한 Sox 유전자 그룹은 다양한 동물 계통에서 빠르게 분화한 최소 40개의 서로 다른 유전자좌가 모여 있습니다. 현재 30개의 Sox 유전자가 확인되었습니다. 이 계열의 유전자들은 진화 과정에서 보존되어 왔으며 동물의 발달 과정에서 중요한 역할을 하는 것으로 밝혀졌습니다. 일부는 성전환을 비롯한 인간 질병에 관여합니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

Sox9 항체 (E-9) 참고문헌:

  1. 새로운 Sry 관련 유전자와 그 생식세포 특이적 발현을 확인했습니다.  |  Osaki, E., et al. 1999. Nucleic Acids Res. 27: 2503-10. PMID: 10359848
  2. 신경 결정에서 Sox 인자의 역할: 진화에서 보존된 신호?  |  Sasai, Y. 2001. Int J Dev Biol. 45: 321-6. PMID: 11291862
  3. HMG 박스 슈퍼패밀리의 조상과 다양성.  |  Laudet, V., et al. 1993. Nucleic Acids Res. 21: 2493-501. PMID: 8506143
  4. 신경교 세포의 새로운 전사 조절 인자인 Sox10.  |  Kuhlbrodt, K., et al. 1998. J Neurosci. 18: 237-50. PMID: 9412504
  5. 인간 SOX14 유전자의 특성화 및 매핑.  |  Arsic, N., et al. 1998. Cytogenet Cell Genet. 83: 139-46. PMID: 9925951

주문정보

제품명카탈로그 번호 단위가격수량관심품목

Sox9 항체 (E-9)

sc-166505
200 µg/ml
$316.00

Sox9 (E-9): m-IgG Fc BP-HRP 번들

sc-528983
200 µg Ab; 10 µg BP
$354.00

Sox9 (E-9): m-IgGκ BP-HRP 번들

sc-521635
200 µg Ab, 40 µg BP
$354.00

Sox9 (E-9): m-IgG2a BP-HRP 번들

sc-547225
200 µg Ab; 10 µg BP
$354.00

Sox9 항체 (E-9) X

sc-166505 X
200 µg/0.1 ml
$316.00

Sox9 항체 (E-9) AC

sc-166505 AC
500 µg/ml, 25% agarose
$416.00

Sox9 항체 (E-9) HRP

sc-166505 HRP
200 µg/ml
$316.00

Sox9 항체 (E-9) FITC

sc-166505 FITC
200 µg/ml
$330.00

Sox9 항체 (E-9) PE

sc-166505 PE
200 µg/ml
$343.00

Sox9 항체 (E-9) Alexa Fluor® 488

sc-166505 AF488
200 µg/ml
$357.00

Sox9 항체 (E-9) Alexa Fluor® 546

sc-166505 AF546
200 µg/ml
$357.00

Sox9 항체 (E-9) Alexa Fluor® 594

sc-166505 AF594
200 µg/ml
$357.00

Sox9 항체 (E-9) Alexa Fluor® 647

sc-166505 AF647
200 µg/ml
$357.00

Sox9 항체 (E-9) Alexa Fluor® 680

sc-166505 AF680
200 µg/ml
$357.00

Sox9 항체 (E-9) Alexa Fluor® 790

sc-166505 AF790
200 µg/ml
$357.00

Sox9 (E-9) 중화펩타이드

sc-166505 P
100 µg/0.5 ml
$68.00

I would like to know at which dilution this antibody Sox9/647 (sc-166505) is used for immunofluorescence assays. Thanks, Jordi

Asked by: Jordi
Santa Cruz Biotechnology tests at a 1:50 dilution for Immunofluorescence.
Answered by: Technical Service
Date published: 2024-05-07

Is this product appropriate for flow cytometry? Have you got citations of people successfully using it?

Asked by: ailsaw
Thank you for your question. Fluorescent conjugates of this antibody can be used for flow cytometry. As of yet, we have not received any published data on flow cytometry use.
Answered by: Tech Support Europe
Date published: 2023-09-22

where is the band area， why mine is at 40~，

Asked by: minch
Thank you for your question. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Jiajun Z
Date published: 2023-07-27

Can the conjugated version of this antibody (546) be used in combination with other secondary antibodies for double-staining? I haven't used many mouse-on-mouse stains and I know that can be problematic but mouse is listed under reactive species.

Asked by: Anonymous
Thank you for your question. We do not recommend using a secondary antibody or binding protein with a primary antibody that is already conjugated. Steric hindrance may negatively influence your results. Most samples and tissues are unproblematic when using a mouse primary antibody and anti-mouse secondary detection. When working with tissues make sure to remove all blood from the sample to prevent the secondary from picking up endogenous IgG. Using a direct conjugate (without a secondary) will circumvent this issue entirely.
Answered by: Technical Support Europe
Date published: 2020-10-28

Has anyone tried with IHC before? I have got mice testes samples which are paraffin fixed. I tried doing it but there isn't any signal.

Asked by: Evaa
Thank you for your question. We have not yet tested this antibody in formalin-fixed paraffin-embedded tissue sections, and so IHC(P) is not a recommended application at this time. We have successfully used the antibody on formalin-fixed cells for IF. In addition, there are several publications citing use of this antibody for IHC staining. For example, PMID's: 25917841 and 30244259.
Answered by: Technical Support
Date published: 2019-02-26

Is this product acceptable for ChIP/ChIP-seq applications?

Asked by: che183
This antibody has not yet been tested in the ChIP or ChIP-seq applications.
Answered by: Technical Support
Date published: 2018-12-21

I used this antibody for IF in rat cartilage cryosections but no staining was observed. Could you please recommend a protocol for this? Which is the best fixative and permeabilization agent? Is antigen retrieval necessary? Thanks.

Asked by: sox9
Thank you for your question. I have provided a link to our recommended protocol for immunofluorescent staining of frozen tissue sections. We recommend fixing slides in cold acetone for 10 minutes. A permeabilization agent should not be necessary if fixing in acetone. If you are using a formalin-based fixative, then permeabilization with Triton X-100 may be needed. I would also recommend reviewing the methods in the following publication on PubMed (PMID: 25917841) which cites use of this Sox-9 Antibody (E-9): sc-166505 for IHC in rat tissue. https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-01-13

What is the recommended fixation method for IF with this Sox-9 (E-9): sc-166505?

Asked by: cjMara
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10ºC methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support 9
Date published: 2017-01-11
  • y_2025, m_8, d_28, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_9
  • loc_ko_KR, sid_166505, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 105ms
  • QUESTIONS, PRODUCT
Rated 1 out of 5 by from Poor astrocyte specificityUsed this antibody for astrocyte-specific labeling and it labeled both neurons and astrocytes. Very bad specificity. IHC 1:200 and 1:1000 concentrations, both had very bad astrocyte labeling.
Date published: 2025-03-13
Rated 1 out of 5 by from Did not work well for WBThis Ab did not work well for neonatal mouse lungs for WB.
Date published: 2024-12-10
Rated 5 out of 5 by from First class service.Express delivery, proper documentation, perfect quality.
Date published: 2022-07-28
Rated 5 out of 5 by from 功能很出色，发货速度快，技术支持很给力！客服答复很快，供货稳定，发货速度快，说明很详细，使用效果很好，技术支持也很给力！会推荐给同事，后期需要还会购买！
Date published: 2022-07-04
Rated 5 out of 5 by from CorrectoFunciona correctamente, dentro de los estandares establecidos
Date published: 2021-08-23
Rated 4 out of 5 by from 不错产品不错, 值得下一次购买哦.....................................
Date published: 2021-08-09
Rated 5 out of 5 by from SOX9 antibodyExcellent antibody for immunofluorescence and immunohistochemistry. Dilution: 1 to 1000. Tested in intestinal tissue
Date published: 2021-03-18
Rated 3 out of 5 by from IF(free sample) used for IF for characterization of tumor cells, No problem for signal detection
Date published: 2020-10-06
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_34
  • loc_ko_KR, sid_166505, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 14ms
  • REVIEWS, PRODUCT
Sox9 항체 (E-9) is rated 4.1 out of 5 by 34.
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_34
  • loc_ko_KR, sid_166505, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 102ms
  • REVIEWS, PRODUCT
001 800-1338-3838
방문 Santa Cruz Animal Health|방문 San Juan Ranch
산타크루즈바이오테크놀러지는 생물의학연구에 필요한 제품을 개발하는 선구자입니다. Toll Free 1-800-457-3801로 연락주세요. Copyright © 2007-2025 Santa Cruz Biotechnology, Inc. 판권소유. “Santa Cruz Biotechnology”, 와 Santa Cruz Biotechnology, Inc. 로고, “Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, 및 San Juan Ranch 로고, “UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”와 “EZ Touch” 는 Santa Cruz Biotechnology, Inc 의 등록된 상표입니다. 모든 상표는 각자 소유주의 소유입니다.