Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SNARK CRISPR/Cas9 KO Plasmid (h): sc-405079
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SNARK CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SNARK genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SNARK Antibody (C-12): sc-374348
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SNARK CRISPR/Cas9 KO Plasmid (h) | sc-405079 | 20 µg | $397.00 |
Overview
NUAK2 encodes SNARK (SNF1/AMPK-related kinase), a serine/threonine kinase in the AMPK-related kinase family that links cellular energy status to adaptive signaling programs. SNARK is activated downstream of LKB1/STK11 and participates in phosphorylation networks that influence cytoskeletal remodeling, cell adhesion and migration, and stress-responsive metabolic regulation. Reported functions connect NUAK2 to pathways governing epithelial–mesenchymal transition, Hippo/YAP-associated transcriptional outputs, and context-dependent control of proliferation and survival. Dysregulated NUAK2/SNARK activity has been associated with cancer biology and invasive phenotypes, supporting its use as a target for mechanistic studies of metabolism–motility coupling.
SNARK CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the NUAK2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the NUAK2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the NUAK2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SNARK protein expression.
This CRISPR knockout system enables efficient generation of NUAK2-deficient cell models for investigation of SNARK signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting NUAK2 exon(s) critical for SNARK function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple NUAK2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SNARK CRISPR/Cas9 KO Plasmid (h) and SNARK CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the NUAK2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SNARK HDR Plasmid (h) and SNARK HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by NUAK2 homology arms to support homology-directed repair at defined NUAK2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.