SENP5 Double Nickase Plasmid (h): sc-404428-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SENP5 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SENP5 Double Nickase Plasmid (h) and SENP5 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SENP5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SENP5 Antibody (3C2): sc-293373
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SENP5 Double Nickase Plasmid (h) | sc-404428-NIC | 20 µg | $410.00 | |||
SENP5 Double Nickase Plasmid (h2) | sc-404428-NIC-2 | 20 µg | $410.00 |
Human SENP5 encodes a SUMO-specific protease that removes SUMO modifications from protein substrates, thereby tuning SUMO-dependent control of protein stability, localization, and macromolecular complex assembly. SENP5 activity contributes to coordination of cell cycle progression and mitotic events, with reported roles in centrosome and nucleolar-associated processes and in maintaining proteostasis during cellular stress. By regulating the balance between SUMOylation and deSUMOylation, SENP5 influences transcriptional programs and DNA damage response signaling that shape cell fitness and genome integrity. Dysregulated SUMO pathway activity, including altered SENP family function, has been linked to proliferative phenotypes and molecular features observed across multiple disease contexts, making SENP5 a useful node for mechanistic studies.
SENP5 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SENP5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SENP5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SENP5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SENP5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.