RuvB Antibody (4G5/1): sc-53436

RuvB Antibody (4G5/1) is a mouse monoclonal IgG1 kappa light chain antibody that detects the RuvB protein of E. coli origin by western blotting (WB) and immunoprecipitation (IP). RuvB (4G5/1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RuvB plays a crucial role in the late stages of homologous recombination and DNA repair, specifically in processing Holliday junctions, which are critical intermediates in these processes. RuvB, along with RuvA and RuvC, is essential for maintaining genomic stability, as RuvB facilitates branch migration and interacts with damaged DNA to promote repair. RuvA enhances the ATPase activity of RuvB, and this interaction is further stimulated by UV irradiation, highlighting RuvB′s importance in responding to DNA damage. RuvB′s ability to induce conformational changes in DNA using ATP is vital for effective DNA repair and recombination, making RuvB a key player in cellular responses to DNA damage and replication stress. Additionally, the RuvABC complex is implicated in the formation of double-strand breaks at stalled replication forks, underscoring RuvB′s significance in maintaining the integrity of the bacterial genome.

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RuvB Antibody (4G5/1) References:

1. The hexameric ring structure of the Escherichia coli RuvB branch migration protein.  |  Chen, YJ., et al. 2002. J Mol Biol. 319: 587-91. PMID: 12054856
2. Crystal structure of the RuvA-RuvB complex: a structural basis for the Holliday junction migrating motor machinery.  |  Yamada, K., et al. 2002. Mol Cell. 10: 671-81. PMID: 12408833
3. Impairment of the DNA binding activity of the TATA-binding protein renders the transcriptional function of Rvb2p/Tih2p, the yeast RuvB-like protein, essential for cell growth.  |  Ohdate, H., et al. 2003. J Biol Chem. 278: 14647-56. PMID: 12576485
4. Uncoupling of the ATPase activity from the branch migration activity of RuvAB protein complexes containing both wild-type and ATPase-defective RuvB proteins.  |  Hishida, T., et al. 2003. Genes Cells. 8: 721-30. PMID: 12940820
5. Direct evidence that a conserved arginine in RuvB AAA+ ATPase acts as an allosteric effector for the ATPase activity of the adjacent subunit in a hexamer.  |  Hishida, T., et al. 2004. Proc Natl Acad Sci U S A. 101: 9573-7. PMID: 15210950
6. Function and regulation of the cyanobacterial genes lexA, recA and ruvB: LexA is critical to the survival of cells facing inorganic carbon starvation.  |  Domain, F., et al. 2004. Mol Microbiol. 53: 65-80. PMID: 15225304
7. Structure-function analysis of the three domains of RuvB DNA motor protein.  |  Ohnishi, T., et al. 2005. J Biol Chem. 280: 30504-10. PMID: 15972826
8. Expression, purification, crystallization and preliminary X-ray analysis of the human RuvB-like protein RuvBL1.  |  Gorynia, S., et al. 2006. Acta Crystallogr Sect F Struct Biol Cryst Commun. 62: 61-6. PMID: 16511264
9. Mycobacterium tuberculosis suppresses host DNA repair to boost its intracellular survival.  |  Liu, S., et al. 2023. Cell Host Microbe. 31: 1820-1836.e10. PMID: 37848028
10. RuvA, RuvB and RuvC proteins: cleaning-up after recombinational repairs in E. coli.  |  Kuzminov, A. 1993. Bioessays. 15: 355-8. PMID: 8393667
11. In vitro reconstitution of the late steps of genetic recombination in E. coli.  |  Eggleston, AK., et al. 1997. Cell. 89: 607-17. PMID: 9160752
12. Formation of RuvABC-Holliday junction complexes in vitro.  |  Davies, AA. and West, SC. 1998. Curr Biol. 8: 725-7. PMID: 9637927

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RuvB Antibody (4G5/1) has 0 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RuvB Antibody (4G5/1) Specifications

Alternate Product Name: RuvB

Product Clone Name: 4G5/1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: RuvB

Epitope Species: E. coli

Product Reactivity/Detection: RuvB

Species Reactivity/Detection: E. coli

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for RuvB Antibody (4G5/1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin