Date published: 2025-9-9

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RUNX1 항체 (DW71): sc-101146

5.0(10)
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데이터 시트
  • RUNX1 항체(DW71) 는 마우스 monoclonal IgG2bκ, 15간행물에 인용, 이며 100 µg/ml으로 제공합니다.
  • human origin의 RUNX1의 전체인 210-311 아미노산을 항원으로 사용하였습니다.
  • WB, IP, IF, IHC(P)와 ELISA으로 mouse, rat와 human origin의 a broad range of RUNX1 isoforms을 검출할것을 권장합니다.
  • See RUNX1 (A-2): sc-365644 for additional antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • RUNX1 (DW71): sc-101146무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • 현재 RUNX1 항체(DW71)에 대해 선호하는 2차 검출 시약의 식별을 아직 완료하지 못했습니다. 이 작업은 진행 중입니다.

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RUNX1 항체(DW71)는 마우스, 쥐 및 인간 유래의 광범위한 RUNX1 이소폼을 WB, IP, IF, IHC(P) 및 ELISA로 검출하는 IgG2b κ 마우스 단일 클론 RUNX1 항체입니다. RUNX1 항체(DW71)는 비접합 항체 형태의 항-RUNX1 항체로 제공됩니다. 포유류 런트 관련 전사인자(RUNX) 계열은 RUNX1(AML-1, PEBP2αB, CBFA2로도 지정), RUNX2(AML-3, PEBP2αA, CBFA1, Osf2로도 지정) 및 RUNX3(AML-2, PEBPαC, CBFA3로도 지정)의 세 멤버로 구성되어 있습니다. RUNX 계열은 세포 분화 및 세포 주기 진행에 관여하는 유전자의 발현을 조절하는 DNA 결합 단백질입니다. RUNX1은 조혈에 관여하며, 인간 백혈병에서 RUNX1의 DNA 결합 도메인을 다른 전사인자 및 코어프레서 분자와 융합하는 염색체 전좌에 의해 표적이 되는 경우가 많습니다. 백혈병 발생에 대한 역할 외에도 RUNX1은 감각 신경세포의 다양화에도 관여합니다. 구체적으로, RUNX1은 축삭 성장을 촉진하고 신경능선 유래 TrkA+ 감각 뉴런에서 선택적으로 발현되며 이동성 신경능선 세포에서 TrkA 전이 활성화를 매개합니다. 대체 스플라이싱은 RUNX1의 여러 이소폼을 생성합니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

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RUNX1 항체 (DW71)

sc-101146
100 µg/ml
$333.00

Hello, is this antibody recognize the N terminal or C terminal of RUNX1?

Asked by: YIZHEN
Thank you for your question. This RUNX1 antibody was raised against recombinant protein mapping within amino acids 210-310 of RUNX1 of human origin. The exact epitope has not been mapped.
Answered by: Tech Service
Date published: 2019-10-10

Does the antibody RUNX1 Antibody (DW71): sc-101146 raise against which part of the Runx1?

Asked by: sunny Zhang
Thank you for your question. Please contact our Technical Service Department and we can assist you by providing an amino acid range within where the epitope is mapping for this antibody.
Answered by: Technical Support
Date published: 2019-01-25

I was wondering which method for antigen retrieval (IHCp) is proposed for this antibody? I tried heated citric acid buffer, varied dilutions of the antibody from 1:50-500 and did not get any signal. Thanks for your help.

Asked by: Friederike Philipp
Thank you for your question. We recommend using one of three methods for antigen retrieval: Heat treatment (recommended method): Place slides in a container and cover with 10 mM sodium citrate buffer, pH 6.0 (sc-294091); or with 50 mM glycine-HCl buffer (sc-29096), pH 3.5, with 0.01% (w/v) EDTA (sc-29092). Heat at 95° C for 5 minutes. Top off with fresh buffer and heat at 95° C for 5 minutes (optimal incubation time may vary for each tissue type). Allow slides to cool in the buffer for approximately 20 minutes. Wash in deionized H2O three times for 2 minutes each. Aspirate excess liquid from slides. Pepsin: Incubate sections for 10–20 minutes in 0.1% Pepsin (sc-476554) in 0.01 N HCl at room temperature. Wash slides several times in deionized H2O. Aspirate excess liquid from slides. Saponin: Incubate sections for 30 minutes in 0.05% Saponin (sc-280079) in deionized H2O at room temperature. Wash at least three times in PBS. Aspirate excess liquid from slides. You can find our complete recommended protocol by following this link: https://www.scbt.com/scbt/de/resources/protocols/immunofluorescence-cell-staining If you are still having trouble with this antibody, please contact our Technical Service by phone or email or by Live Chat and if we cannot resolve the issue, we will provide an appropriate replacement free of charge.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-22

I need to use an antibody to detect the protein in samples of porcine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 7
Date published: 2017-02-15
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Rated 5 out of 5 by from Good for IHC staining on fixed mouse muscleThe antibody works well when I do immunostaining on sections cut from fixed mouse muscles.
Date published: 2023-05-24
Rated 5 out of 5 by from Good specificityFairly clean Western Blot banding and nuclear localization in IF and IHC.
Date published: 2017-01-18
Rated 5 out of 5 by from good functionIt worked very well and the background was clean. Happy to use it.
Date published: 2016-11-24
Rated 5 out of 5 by from Used successfully in WBUsed successfully in WB. Published - See PubMed ID 23442798.
Date published: 2015-09-29
Rated 5 out of 5 by from Strong band in WB in human HAFTL preStrong band in WB in human HAFTL pre-B cells and RAW264.7 cells. -SCBT Publication Review
Date published: 2015-04-23
Rated 5 out of 5 by from Good publishable data in WB in human AsPc1Good publishable data in WB in human AsPc1, HeLa, RKO, HEK293, and MSTO-211H cell lines. -SCBT Publication Review
Date published: 2015-04-23
Rated 5 out of 5 by from Robust signal in WB in SHRobust signal in WB in SH-SY5Y, HeLa, HL-60, THP-1 and U-937 nuclear extracts. -SCBT QC
Date published: 2015-04-01
Rated 5 out of 5 by from Worked well in ChiP and IP in mouse and humanWorked well in ChiP and IP in mouse and human 2D2 mouse T cell, Resting (Pan) CD4 T cells, Human CD4 T cell. -SCBT Publication Review
Date published: 2015-02-12
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RUNX1 항체 (DW71) is rated 5.0 out of 5 by 10.
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