Rpd3 Antibody (G-9): sc-514160

Rpd3 Antibody (G-9) is a mouse monoclonal IgG2a kappa light chain antibody that detects Rpd3 protein of Saccharomyces cerevisiae origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Rpd3 antibody (G-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Rpd3 protein plays a crucial role in chromatin remodeling, which is essential for the regulation of gene expression. Specifically, Rpd3 functions as a histone deacetylase, reversing the acetylation of histones that is necessary for transcriptional activation. This deacetylation process is vital because this leads to a more compact chromatin structure, thereby inhibiting the accessibility of transcription factors to DNA and resulting in transcriptional silencing. Rpd3 interacts with several key proteins, including Sin3, which recruits Rpd3 to specific gene promoters, thereby facilitating the repression of target genes. Understanding Rpd3 dynamics and interactions is important for elucidating the mechanisms of transcriptional regulation and broader implications for cellular function and development in yeast.

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Rpd3 Antibody (G-9) References:

1. HDA1 and HDA3 are components of a yeast histone deacetylase (HDA) complex.  |  Carmen, AA., et al. 1996. J Biol Chem. 271: 15837-44. PMID: 8663039
2. Opening the way to gene activity.  |  Pennisi, E. 1997. Science. 275: 155-7. PMID: 8999545
3. A large protein complex containing the yeast Sin3p and Rpd3p transcriptional regulators.  |  Kasten, MM., et al. 1997. Mol Cell Biol. 17: 4852-8. PMID: 9234741

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Rpd3 Antibody (G-9) has 2 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Rpd3 Antibody (G-9) Specifications

Alternate Product Name: Rpd3

Product Clone Name: G-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 78-95

Epitope Target: Rpd3

Epitope Species: Saccharomyces cerevisiae

Product Reactivity/Detection: Rpd3

Species Reactivity/Detection: Saccharomyces cerevisiae

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for Rpd3 Antibody (G-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin