RNGTT Antibody (H-3): sc-514128
- RNGTT Antibody (H-3) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against amino acids 211-510 mapping within an internal region of RNGTT of human origin
- Anti-RNGTT Antibody (H-3) is recommended for detection of RNGTT of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-RNGTT Antibody (H-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for RNGTT Antibody (H-3) for WB applications. These reagents are now offered in bundles with RNGTT Antibody (H-3) (see ordering information below).
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RNGTT Antibody (H-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects RNGTT in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RNGTT (H-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RNGTT, also known as RNA guanylyltransferase and 5′-phosphatase, HCE, HCE1, hCAP, or CAP1A, is a 597 amino acid protein that plays a crucial role in mRNA processing by acting as a bifunctional mRNA-capping enzyme. RNGTT is primarily located in the nucleus and is expressed in various tissues, including kidney, lung, heart, brain, liver, testis, skin, and muscle, where RNGTT exists as four alternatively spliced isoforms. RNGTT′s dual enzymatic activities—RNA 5′-triphosphatase at its N-terminus and mRNA guanylyltransferase at its C-terminus—are essential for the initial steps of mRNA cap formation, which involves removing a gamma-phosphate from nascent mRNA and transferring the phospho residue to the 5′-diphosphate terminus. This capping process is vital for mRNA stability, nuclear export, and translation efficiency, highlighting RNGTT′s importance in gene expression regulation. The gene encoding RNGTT is located on human chromosome 6, which encompasses 170 million base pairs and constitutes nearly 6% of the human genome.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RNGTT Antibody (H-3) References:
- Myc Regulation of mRNA Cap Methylation. | Cowling, VH. and Cole, MD. 2010. Genes Cancer. 1: 576-579. PMID: 21170289
- HERV-E-mediated modulation of PLA2G4A transcription in urothelial carcinoma. | Gosenca, D., et al. 2012. PLoS One. 7: e49341. PMID: 23145155
- Human cap methyltransferase (RNMT) N-terminal non-catalytic domain mediates recruitment to transcription initiation sites. | Aregger, M. and Cowling, VH. 2013. Biochem J. 455: 67-73. PMID: 23863084
- RAM function is dependent on Kapβ2-mediated nuclear entry. | Gonatopoulos-Pournatzis, T. and Cowling, VH. 2014. Biochem J. 457: 473-84. PMID: 24200467
- Identification of potential drug targets for tuberous sclerosis complex by synthetic screens combining CRISPR-based knockouts with RNAi. | Housden, BE., et al. 2015. Sci Signal. 8: rs9. PMID: 26350902
- c-Myc deregulation induces mRNA capping enzyme dependency. | Lombardi, O., et al. 2016. Oncotarget. 7: 82273-82288. PMID: 27756891
- RNA-binding proteins and heat-shock protein 90 are constituents of the cytoplasmic capping enzyme interactome. | Trotman, JB., et al. 2018. J Biol Chem. 293: 16596-16607. PMID: 30166341
- A novel RNA pol II CTD interaction site on the mRNA capping enzyme is essential for its allosteric activation. | Bage, MG., et al. 2021. Nucleic Acids Res. 49: 3109-3126. PMID: 33684220
- A novel and independent survival prognostic model for OSCC: the functions and prognostic values of RNA-binding proteins. | Wang, S., et al. 2024. Eur Arch Otorhinolaryngol. 281: 397-409. PMID: 37656222
- Redundant pathways for removal of defective RNA polymerase II complexes at a promoter-proximal pause checkpoint. | Blears, D., et al. 2024. Mol Cell. 84: 4790-4807.e11. PMID: 39504960
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RNGTT Antibody (H-3) | sc-514128 | 200 µg/ml | $322.00 | |||
RNGTT Antibody (H-3): m-IgG Fc BP-HRP Bundle | sc-531038 | 200 µg Ab; 10 µg BP | $361.00 | |||
RNGTT Antibody (H-3): m-IgGκ BP-HRP Bundle | sc-524616 | 200 µg Ab, 40 µg BP | $361.00 | |||
RNGTT Antibody (H-3) AC | sc-514128 AC | 500 µg/ml, 25% agarose | $424.00 | |||
RNGTT Antibody (H-3) HRP | sc-514128 HRP | 200 µg/ml | $322.00 | |||
RNGTT Antibody (H-3) FITC | sc-514128 FITC | 200 µg/ml | $336.00 | |||
RNGTT Antibody (H-3) PE | sc-514128 PE | 200 µg/ml | $349.00 | |||
RNGTT Antibody (H-3) Alexa Fluor® 488 | sc-514128 AF488 | 200 µg/ml | $364.00 | |||
RNGTT Antibody (H-3) Alexa Fluor® 546 | sc-514128 AF546 | 200 µg/ml | $364.00 | |||
RNGTT Antibody (H-3) Alexa Fluor® 594 | sc-514128 AF594 | 200 µg/ml | $364.00 | |||
RNGTT Antibody (H-3) Alexa Fluor® 647 | sc-514128 AF647 | 200 µg/ml | $364.00 | |||
RNGTT Antibody (H-3) Alexa Fluor® 680 | sc-514128 AF680 | 200 µg/ml | $364.00 | |||
RNGTT Antibody (H-3) Alexa Fluor® 790 | sc-514128 AF790 | 200 µg/ml | $364.00 |